Evidence for secretory pathway localization of a voltage-dependent anion channel isoform

Reinhard Buettner, Georg Papoutsoglou, Eliana Scemes, David C. Spray, Rolf Dermietzel

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Voltage-dependent anion channels (VDACs) are pore-forming proteins (porins) that form the major pathway for movement of adenine nucleotides through the outer mitochondrial membrane. Electrophysiological studies indicate that VDAC-like channel activity is also prevalent in the cell membranes of many mammalian cells. However, the multitopological localization of porins outside the mitochondrion has remained an extremely controversial issue. Herein, we show that usage of two alternative first exons of the murine VDAC-1 gene leads to expression of two porins differing within their N termini. One porin (plasmalemmal VDAC-1) harboring a hydrophobic leader peptide is primarily targeted through the Golgi apparatus to the cell membrane. In contrast, the second isoform lacking the N-terminal leader (mitochondrial VDAC-1) is translocated more efficiently into the outer mitochondrial membrane. Thus, our data provide unique genetic evidence in favor of a multitopological localization of a mitochondrial porin.

Original languageEnglish (US)
Pages (from-to)3201-3206
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number7
DOIs
StatePublished - Mar 28 2000

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Voltage-Dependent Anion Channels
Porins
Secretory Pathway
Voltage-Dependent Anion Channel 1
Protein Isoforms
Mitochondrial Membranes
Cell Membrane
Adenine Nucleotides
Golgi Apparatus
Protein Sorting Signals
Exons
Mitochondria
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Evidence for secretory pathway localization of a voltage-dependent anion channel isoform. / Buettner, Reinhard; Papoutsoglou, Georg; Scemes, Eliana; Spray, David C.; Dermietzel, Rolf.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 7, 28.03.2000, p. 3201-3206.

Research output: Contribution to journalArticle

Buettner, Reinhard ; Papoutsoglou, Georg ; Scemes, Eliana ; Spray, David C. ; Dermietzel, Rolf. / Evidence for secretory pathway localization of a voltage-dependent anion channel isoform. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 7. pp. 3201-3206.
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