Evidence for NH2- and COOH-terminal interactions in rat 6-phosphofructo- 2-kinase/fructose-2,6-bisphosphatase

K. Lin, I. J. Kurland, L. Li, Hwan Lee Yong Hwan Lee, D. Okar, J. F. Marecek, S. J. Pilkis

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The pH kinetic behavior of several rat fructose-2,6-bisphosphatase forms was analyzed. The bisphosphatase maximal velocity of the hepatic 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase was optimal at pH 5, but decreased to 12% of the optimal value in the pH range 7.0-7.5. This decrease depended on deprotonation of a group with a pK of 5.7. In contrast, the separate bisphosphatase domain, a 30-amino acid COOH-terminal truncated form (CT30) of the liver enzyme, and the skeletal muscle bifunctional enzyme exhibited pH-insensitive maximal velocities which were 5-10-fold higher than that of the bisphosphatase of the liver bifunctional enzyme at pH 7.0-7.5. The pK values of the C-2 and C-6 phosphoryl groups were 6.0 and 5.75, respectively, as determined by 31P NMR. Analysis of log k(cat)/K(m) versus pH profiles revealed two pK values, one at 6.1, which probably is a substrate pK, and the other at 8.4, which represents an enzyme group. Protein kinase- catalyzed phosphorylation of the liver isoform activated the bisphosphatase, and the pK of the group seen in the k(cat) profile was increased from 5.7 to 6.4. However, phosphorylation of the CT30 mutant had no effect on the bisphosphatase. The data indicate that NH2- and COOH-terminal interactions in the liver bifunctional enzyme affect the pH dependence of the fructose- 2,6-bisphosphatase and its activation by phosphorylation.

Original languageEnglish (US)
Pages (from-to)16953-16960
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number24
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Lin, K., Kurland, I. J., Li, L., Yong Hwan Lee, H. L., Okar, D., Marecek, J. F., & Pilkis, S. J. (1994). Evidence for NH2- and COOH-terminal interactions in rat 6-phosphofructo- 2-kinase/fructose-2,6-bisphosphatase. Journal of Biological Chemistry, 269(24), 16953-16960.