Evidence for lipid peroxidation by paraquat in the perfused rat lung

T. K. Aldrich, A. B. Fisher, E. Cadenas, B. Chance

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31 Scopus citations

Abstract

In order to evaluate the effect of paraquat on oxidative radical reactions in the lung, we studied MDA production and chemiluminescence (spontaneous and tBuOOH-induced) in the isolated rat lung. After 2 hr of perfusion with 3.0 mM paraquat, MDA content in lung homogenates was 16±7 nmol/gm dry weight higher than in control lungs (mean ±S.E., n = 7, p < 0.05 by paired t test); during 30 min of perfusion, malondialdehyde efflux was 33±15 nmol/gm dry weight higher than in control perfusates (n = 6, p < 0.05). Spontaneous chemiluminescence was not augmented by 2 hr of perfusion with concentratons of paraquat ranging from 0.75 to 6.0 mM. On the other hand, tBuOOH-induced chemiluminescence was 17%±3 higher immediately after the addition of hydroperoxidase and reached a 16%±6 higher plateau for the paraquat-perfused lungs than for control lungs (n = 10, p < 0.05). Spectral analysis of the light emitted during induced chemiluminescence demonstrated peak intensity between 630 and 730 nm for both control and paraquat-treated lungs. Increased MDA production and increased induced chemiluminescence indicate that perfusion with paraquat enhances lipid peroxidation in the isolated rat lung.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume101
Issue number1
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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