In order to evaluate the effect of paraquat on oxidative radical reactions in the lung, we studied MDA production and chemiluminescence (spontaneous and tBuOOH-induced) in the isolated rat lung. After 2 hr of perfusion with 3.0 mM paraquat, MDA content in lung homogenates was 16±7 nmol/gm dry weight higher than in control lungs (mean ±S.E., n = 7, p < 0.05 by paired t test); during 30 min of perfusion, malondialdehyde efflux was 33±15 nmol/gm dry weight higher than in control perfusates (n = 6, p < 0.05). Spontaneous chemiluminescence was not augmented by 2 hr of perfusion with concentratons of paraquat ranging from 0.75 to 6.0 mM. On the other hand, tBuOOH-induced chemiluminescence was 17%±3 higher immediately after the addition of hydroperoxidase and reached a 16%±6 higher plateau for the paraquat-perfused lungs than for control lungs (n = 10, p < 0.05). Spectral analysis of the light emitted during induced chemiluminescence demonstrated peak intensity between 630 and 730 nm for both control and paraquat-treated lungs. Increased MDA production and increased induced chemiluminescence indicate that perfusion with paraquat enhances lipid peroxidation in the isolated rat lung.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Laboratory and Clinical Medicine|
|Publication status||Published - Jan 1 1983|
ASJC Scopus subject areas
- Pathology and Forensic Medicine