Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products

Katarzyna Wieczorowska-Tobis, Renata Brelinska, Janusz Witowski, Jutta Passlick-Deetjen, Thomas P. Schaub, Holger Schilling, Andrzej Breborowicz

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

◆ Background: Acidic pH and the presence of glucose degradation products (GDP) are believed to compromise the biocompatibility of peritoneal dialysis fluids (PDF).The present study examines the effects of long-term exposure to GDP and low pH by comparing conventional PDF and a new, neutral pH, low GDP solution. ◆ Methods: All experiments were performed using a chronic infusion model of dialysis in nonuremic rats. The animals were treated for 6 weeks with 2 daily injections of 4.25% glucose-containing PDF.The following PDF were tested: CAPD3 (single-chamber bag, low pH, high GDP), CAPD3 pH 7.4 (single-chamber bag, neutral pH, high GDP), CAPD3-Balance (double-chamber bag, neutral pH, low GDP). All test solutions were obtained from Fresenius Medical Care, Bad Homburg, Germany. ◆ Results: After 6 weeks of exposure, peritoneal permeability to water, urea, creatinine, glucose, and sodium, assessed by peritoneal equilibration test, was similar in all groups. However, compared to other PDF, dialysis with CAPD3-Balance was associated with reduced concentrations of protein and hyaluronan in the dialysate, decreased peritoneal eosinophilia, and reduced dialysate levels of chemokines CCL2/MCP-1 and CCL5/RANTES. Morphologic changes in the peritoneal membrane of CAPD3-Balance-treated animals were much less pronounced and included reduced vascular density, preservation of the mesothelial monolayer and intercellular junction, and no reduplication of the submesothelial basement membrane. ◆ Conclusions: A new generation of PDF with physiologic pH and low GDP level produce less irritation to the peritoneal membrane and better preserve its structural integrity. This effect seems to be related predominantly to minimized GDP concentrations.

Original languageEnglish (US)
Pages (from-to)48-57
Number of pages10
JournalPeritoneal Dialysis International
Volume24
Issue number1
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Ascitic Fluid
Glucose
Peritoneal Dialysis
Membranes
Dialysis Solutions
Dialysis
Chemokine CCL5
Intercellular Junctions
Chemokine CCL2
Eosinophilia
Hyaluronic Acid
Basement Membrane
Germany
Blood Vessels
Urea
Permeability
Creatinine
Sodium
Injections
Water

Keywords

  • Animal models
  • Biocompatibility
  • Glucose degradation products
  • Peritoneal dialysis fluids
  • Peritoneal Eosinophilia

ASJC Scopus subject areas

  • Nephrology

Cite this

Wieczorowska-Tobis, K., Brelinska, R., Witowski, J., Passlick-Deetjen, J., Schaub, T. P., Schilling, H., & Breborowicz, A. (2004). Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products. Peritoneal Dialysis International, 24(1), 48-57.

Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products. / Wieczorowska-Tobis, Katarzyna; Brelinska, Renata; Witowski, Janusz; Passlick-Deetjen, Jutta; Schaub, Thomas P.; Schilling, Holger; Breborowicz, Andrzej.

In: Peritoneal Dialysis International, Vol. 24, No. 1, 01.2004, p. 48-57.

Research output: Contribution to journalArticle

Wieczorowska-Tobis, K, Brelinska, R, Witowski, J, Passlick-Deetjen, J, Schaub, TP, Schilling, H & Breborowicz, A 2004, 'Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products', Peritoneal Dialysis International, vol. 24, no. 1, pp. 48-57.
Wieczorowska-Tobis K, Brelinska R, Witowski J, Passlick-Deetjen J, Schaub TP, Schilling H et al. Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products. Peritoneal Dialysis International. 2004 Jan;24(1):48-57.
Wieczorowska-Tobis, Katarzyna ; Brelinska, Renata ; Witowski, Janusz ; Passlick-Deetjen, Jutta ; Schaub, Thomas P. ; Schilling, Holger ; Breborowicz, Andrzej. / Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products. In: Peritoneal Dialysis International. 2004 ; Vol. 24, No. 1. pp. 48-57.
@article{65b692db2f9c4f4e8118901979317487,
title = "Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products",
abstract = "◆ Background: Acidic pH and the presence of glucose degradation products (GDP) are believed to compromise the biocompatibility of peritoneal dialysis fluids (PDF).The present study examines the effects of long-term exposure to GDP and low pH by comparing conventional PDF and a new, neutral pH, low GDP solution. ◆ Methods: All experiments were performed using a chronic infusion model of dialysis in nonuremic rats. The animals were treated for 6 weeks with 2 daily injections of 4.25{\%} glucose-containing PDF.The following PDF were tested: CAPD3 (single-chamber bag, low pH, high GDP), CAPD3 pH 7.4 (single-chamber bag, neutral pH, high GDP), CAPD3-Balance (double-chamber bag, neutral pH, low GDP). All test solutions were obtained from Fresenius Medical Care, Bad Homburg, Germany. ◆ Results: After 6 weeks of exposure, peritoneal permeability to water, urea, creatinine, glucose, and sodium, assessed by peritoneal equilibration test, was similar in all groups. However, compared to other PDF, dialysis with CAPD3-Balance was associated with reduced concentrations of protein and hyaluronan in the dialysate, decreased peritoneal eosinophilia, and reduced dialysate levels of chemokines CCL2/MCP-1 and CCL5/RANTES. Morphologic changes in the peritoneal membrane of CAPD3-Balance-treated animals were much less pronounced and included reduced vascular density, preservation of the mesothelial monolayer and intercellular junction, and no reduplication of the submesothelial basement membrane. ◆ Conclusions: A new generation of PDF with physiologic pH and low GDP level produce less irritation to the peritoneal membrane and better preserve its structural integrity. This effect seems to be related predominantly to minimized GDP concentrations.",
keywords = "Animal models, Biocompatibility, Glucose degradation products, Peritoneal dialysis fluids, Peritoneal Eosinophilia",
author = "Katarzyna Wieczorowska-Tobis and Renata Brelinska and Janusz Witowski and Jutta Passlick-Deetjen and Schaub, {Thomas P.} and Holger Schilling and Andrzej Breborowicz",
year = "2004",
month = "1",
language = "English (US)",
volume = "24",
pages = "48--57",
journal = "Peritoneal Dialysis International",
issn = "0896-8608",
publisher = "Multimed Inc.",
number = "1",

}

TY - JOUR

T1 - Evidence for less irritation to the peritoneal membrane in rats dialyzed with solutions low in glucose degradation products

AU - Wieczorowska-Tobis, Katarzyna

AU - Brelinska, Renata

AU - Witowski, Janusz

AU - Passlick-Deetjen, Jutta

AU - Schaub, Thomas P.

AU - Schilling, Holger

AU - Breborowicz, Andrzej

PY - 2004/1

Y1 - 2004/1

N2 - ◆ Background: Acidic pH and the presence of glucose degradation products (GDP) are believed to compromise the biocompatibility of peritoneal dialysis fluids (PDF).The present study examines the effects of long-term exposure to GDP and low pH by comparing conventional PDF and a new, neutral pH, low GDP solution. ◆ Methods: All experiments were performed using a chronic infusion model of dialysis in nonuremic rats. The animals were treated for 6 weeks with 2 daily injections of 4.25% glucose-containing PDF.The following PDF were tested: CAPD3 (single-chamber bag, low pH, high GDP), CAPD3 pH 7.4 (single-chamber bag, neutral pH, high GDP), CAPD3-Balance (double-chamber bag, neutral pH, low GDP). All test solutions were obtained from Fresenius Medical Care, Bad Homburg, Germany. ◆ Results: After 6 weeks of exposure, peritoneal permeability to water, urea, creatinine, glucose, and sodium, assessed by peritoneal equilibration test, was similar in all groups. However, compared to other PDF, dialysis with CAPD3-Balance was associated with reduced concentrations of protein and hyaluronan in the dialysate, decreased peritoneal eosinophilia, and reduced dialysate levels of chemokines CCL2/MCP-1 and CCL5/RANTES. Morphologic changes in the peritoneal membrane of CAPD3-Balance-treated animals were much less pronounced and included reduced vascular density, preservation of the mesothelial monolayer and intercellular junction, and no reduplication of the submesothelial basement membrane. ◆ Conclusions: A new generation of PDF with physiologic pH and low GDP level produce less irritation to the peritoneal membrane and better preserve its structural integrity. This effect seems to be related predominantly to minimized GDP concentrations.

AB - ◆ Background: Acidic pH and the presence of glucose degradation products (GDP) are believed to compromise the biocompatibility of peritoneal dialysis fluids (PDF).The present study examines the effects of long-term exposure to GDP and low pH by comparing conventional PDF and a new, neutral pH, low GDP solution. ◆ Methods: All experiments were performed using a chronic infusion model of dialysis in nonuremic rats. The animals were treated for 6 weeks with 2 daily injections of 4.25% glucose-containing PDF.The following PDF were tested: CAPD3 (single-chamber bag, low pH, high GDP), CAPD3 pH 7.4 (single-chamber bag, neutral pH, high GDP), CAPD3-Balance (double-chamber bag, neutral pH, low GDP). All test solutions were obtained from Fresenius Medical Care, Bad Homburg, Germany. ◆ Results: After 6 weeks of exposure, peritoneal permeability to water, urea, creatinine, glucose, and sodium, assessed by peritoneal equilibration test, was similar in all groups. However, compared to other PDF, dialysis with CAPD3-Balance was associated with reduced concentrations of protein and hyaluronan in the dialysate, decreased peritoneal eosinophilia, and reduced dialysate levels of chemokines CCL2/MCP-1 and CCL5/RANTES. Morphologic changes in the peritoneal membrane of CAPD3-Balance-treated animals were much less pronounced and included reduced vascular density, preservation of the mesothelial monolayer and intercellular junction, and no reduplication of the submesothelial basement membrane. ◆ Conclusions: A new generation of PDF with physiologic pH and low GDP level produce less irritation to the peritoneal membrane and better preserve its structural integrity. This effect seems to be related predominantly to minimized GDP concentrations.

KW - Animal models

KW - Biocompatibility

KW - Glucose degradation products

KW - Peritoneal dialysis fluids

KW - Peritoneal Eosinophilia

UR - http://www.scopus.com/inward/record.url?scp=3042734259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042734259&partnerID=8YFLogxK

M3 - Article

C2 - 15104336

AN - SCOPUS:3042734259

VL - 24

SP - 48

EP - 57

JO - Peritoneal Dialysis International

JF - Peritoneal Dialysis International

SN - 0896-8608

IS - 1

ER -