Evidence for human CD4+ T cells in the CD1-restricted repertoire

Derivation of mycobacteria-reactive T cells from leprosy lesions

Peter A. Sieling, Maria Teresa Ochoa, Denis Jullien, David S. Leslie, Shereen Sabet, Jean Pierre Rosat, Anne E. Burdick, Rea Thomas H, Michael B. Brenner, Steven A. Porcelli, Robert L. Modlin

Research output: Contribution to journalArticle

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Abstract

Both the CD4-CD8- (double negative) and CD4-CD8+ T cell lineages have been shown to contain T cells which recognize microbial lipid and glycolipid Ags in the context of human CD1 molecules. To determine whether T cells expressing the CD4 coreceptor could recognize Ag in the context of CD1, we derived CD4+ T cell lines from the lesions of leprosy patients. We identified three CD4+ Mycobacterium leprae-reactive, CD1-restricted T cell lines: two CD1b restricted and one CD1c restricted. These T cell lines recognize mycobacterial Ags, one of which has not been previously described for CD1-restricted T cells. The response of CD4+ CD1-restricted T cells, unlike MHC class II-restricted T cells, was not inhibited by anti-CD4 mAb, suggesting that the CD4 coreceptor does not impact positive or negative selection of CD1-restricted T cells. The CD4+ CD1-restricted T cell lines produced IFN-γ and GM-CSF, the Th1 pattern of cytokines required for cell- mediated immunity against intracellular pathogens, but no detectable IL-4. The existence of CD4+ CD1-restricted T cells that produce a Th1 cytokine pattern suggests a contributory role in immunity to mycobacterial infection.

Original languageEnglish (US)
Pages (from-to)4790-4796
Number of pages7
JournalJournal of Immunology
Volume164
Issue number9
StatePublished - May 1 2000

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Leprosy
Mycobacterium
T-Lymphocytes
Cell Line
Cytokines
Mycobacterium leprae
Glycolipids
Cell Lineage
Granulocyte-Macrophage Colony-Stimulating Factor
Cellular Immunity
Interleukin-4
Immunity
Lipids

ASJC Scopus subject areas

  • Immunology

Cite this

Sieling, P. A., Ochoa, M. T., Jullien, D., Leslie, D. S., Sabet, S., Rosat, J. P., ... Modlin, R. L. (2000). Evidence for human CD4+ T cells in the CD1-restricted repertoire: Derivation of mycobacteria-reactive T cells from leprosy lesions. Journal of Immunology, 164(9), 4790-4796.

Evidence for human CD4+ T cells in the CD1-restricted repertoire : Derivation of mycobacteria-reactive T cells from leprosy lesions. / Sieling, Peter A.; Ochoa, Maria Teresa; Jullien, Denis; Leslie, David S.; Sabet, Shereen; Rosat, Jean Pierre; Burdick, Anne E.; Thomas H, Rea; Brenner, Michael B.; Porcelli, Steven A.; Modlin, Robert L.

In: Journal of Immunology, Vol. 164, No. 9, 01.05.2000, p. 4790-4796.

Research output: Contribution to journalArticle

Sieling, PA, Ochoa, MT, Jullien, D, Leslie, DS, Sabet, S, Rosat, JP, Burdick, AE, Thomas H, R, Brenner, MB, Porcelli, SA & Modlin, RL 2000, 'Evidence for human CD4+ T cells in the CD1-restricted repertoire: Derivation of mycobacteria-reactive T cells from leprosy lesions', Journal of Immunology, vol. 164, no. 9, pp. 4790-4796.
Sieling PA, Ochoa MT, Jullien D, Leslie DS, Sabet S, Rosat JP et al. Evidence for human CD4+ T cells in the CD1-restricted repertoire: Derivation of mycobacteria-reactive T cells from leprosy lesions. Journal of Immunology. 2000 May 1;164(9):4790-4796.
Sieling, Peter A. ; Ochoa, Maria Teresa ; Jullien, Denis ; Leslie, David S. ; Sabet, Shereen ; Rosat, Jean Pierre ; Burdick, Anne E. ; Thomas H, Rea ; Brenner, Michael B. ; Porcelli, Steven A. ; Modlin, Robert L. / Evidence for human CD4+ T cells in the CD1-restricted repertoire : Derivation of mycobacteria-reactive T cells from leprosy lesions. In: Journal of Immunology. 2000 ; Vol. 164, No. 9. pp. 4790-4796.
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