Evidence against insulin synthesis by IM-9 lymphocytes

W. A. Bauman, Victor Bernard Hatcher, M. A. Levitt, R. S. Yalow

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.

Original languageEnglish (US)
Pages (from-to)182-184
Number of pages3
JournalDiabetes
Volume31
Issue number2
StatePublished - 1982

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Lymphocytes
Insulin
Cell Size
Culture Media
Ethanol
Cell Culture Techniques
Acids
octadecylsilica

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Bauman, W. A., Hatcher, V. B., Levitt, M. A., & Yalow, R. S. (1982). Evidence against insulin synthesis by IM-9 lymphocytes. Diabetes, 31(2), 182-184.

Evidence against insulin synthesis by IM-9 lymphocytes. / Bauman, W. A.; Hatcher, Victor Bernard; Levitt, M. A.; Yalow, R. S.

In: Diabetes, Vol. 31, No. 2, 1982, p. 182-184.

Research output: Contribution to journalArticle

Bauman, WA, Hatcher, VB, Levitt, MA & Yalow, RS 1982, 'Evidence against insulin synthesis by IM-9 lymphocytes', Diabetes, vol. 31, no. 2, pp. 182-184.
Bauman WA, Hatcher VB, Levitt MA, Yalow RS. Evidence against insulin synthesis by IM-9 lymphocytes. Diabetes. 1982;31(2):182-184.
Bauman, W. A. ; Hatcher, Victor Bernard ; Levitt, M. A. ; Yalow, R. S. / Evidence against insulin synthesis by IM-9 lymphocytes. In: Diabetes. 1982 ; Vol. 31, No. 2. pp. 182-184.
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