Evidence against insulin synthesis by IM-9 lymphocytes

W. A. Bauman; V. B. Hatcher; M. A. Levitt; R. S. Yalow

doi:10.2337/diab.31.2.182

Evidence against insulin synthesis by IM-9 lymphocytes

W. A. Bauman, V. B. Hatcher, M. A. Levitt, R. S. Yalow

Biochemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.

Original language	English (US)
Pages (from-to)	182-184
Number of pages	3
Journal	Diabetes
Volume	31
Issue number	2
DOIs	https://doi.org/10.2337/diab.31.2.182
State	Published - 1982

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism

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10.2337/diab.31.2.182

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title = "Evidence against insulin synthesis by IM-9 lymphocytes",

abstract = "Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.",

author = "Bauman, {W. A.} and Hatcher, {V. B.} and Levitt, {M. A.} and Yalow, {R. S.}",

year = "1982",

doi = "10.2337/diab.31.2.182",

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AU - Hatcher, V. B.

AU - Levitt, M. A.

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Y1 - 1982

N2 - Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.

AB - Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1% of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.

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Evidence against insulin synthesis by IM-9 lymphocytes

Abstract

ASJC Scopus subject areas

UN SDGs

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