Evaluation of the relationship between the intra- and extramitochondrial [ATP]/[ADP] ratios using phosphoenolpyruvate carboxykinase

D. F. Wilson, M. Erecinska, Vern L. Schramm

Research output: Contribution to journalArticle

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Abstract

The ratio of free ATP to free ADP in the mitochondrial matrix ([ATP(f)]/[ADP(f)]) has been measured in suspensions of isolated mitochondria under conditions of active phosphorylation of extramitochondrial ADP. These measurements utilized phosphoenolpyruvate carboxykinase which is present in the matrix of mitochondria from the livers of guinea pigs, chickens, and pigeons. Mitochondria isolated from these sources also contain nucleoside diphosphate kinase, malate dehydrogenase, and glutamate dehydrogenase or 3-OH-butyrate dehydrogenase. Together these enzymes catalyze the synthesis of phosphoenolpyruvate and CO2 from oxaloacetate with oxidative phosphorylation as an energy source. These reactions have been shown to be fully reversible in suspensions of mitochondria isolated from the above sources. With oxidative phosphorylation as the source of ATP, phosphoenolpyruvate was synthesized from malate and conversely addition of phosphoenolpyruvate, ADP, and CO2 led to synthesis of malate and ATP. The forward and reverse reactions were allowed to continue until the rate of change of metabolite concentrations was minimal and then the latter were measured. The intramitochondrial [Mg-ATP(f)]/[MgADP(f)] was calculated from the equilibrium constants for the reactions and the measured steady state concentrations of the metabolites in both the intra- and extramitochondrial spaces. The value of the intramitochondrial [MgATP(f)]/[MgADP(f)] was found to exceed the extramitochondrial value (adjusted to the same free Mg2+ concentration) by a factor (±S.E.) of 0.83 ± 0.22 (n = 17) for the forward reaction and 1.81 ± 0.54 (n = 11) for the reverse reaction. It is concluded that the adenine nucleotide translocase catalyzes electroneutral exchange of ATP for ADP and that this reaction does not contribute significantly to the energetics of mitochondrial oxidative phosphorylation.

Original languageEnglish (US)
Pages (from-to)10464-10473
Number of pages10
JournalJournal of Biological Chemistry
Volume258
Issue number17
StatePublished - 1983
Externally publishedYes

Fingerprint

Phosphoenolpyruvate
Adenosine Diphosphate
Adenosine Triphosphate
Mitochondria
Oxidative Phosphorylation
Metabolites
Suspensions
Nucleoside-Diphosphate Kinase
ATP Translocases Mitochondrial ADP
Oxaloacetic Acid
Glutamate Dehydrogenase
Malate Dehydrogenase
Phosphorylation
Butyrates
Liver Mitochondrion
Columbidae
Equilibrium constants
Liver
Chickens
Oxidoreductases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Evaluation of the relationship between the intra- and extramitochondrial [ATP]/[ADP] ratios using phosphoenolpyruvate carboxykinase. / Wilson, D. F.; Erecinska, M.; Schramm, Vern L.

In: Journal of Biological Chemistry, Vol. 258, No. 17, 1983, p. 10464-10473.

Research output: Contribution to journalArticle

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