Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis

P. D. Brown, Claudia Gravekamp, D. G. Carrington, H. Van De Kemp, R. A. Hartskeerl, C. N. Edwards, C. O R Everard, W. J. Terpstra, P. N. Levett

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospirosis were examined by PCR, culture or serology. Samples from 44 (62%) patients with the diagnosis of leptospirosis were positive by PCR as compared to 34 (48%) by culture. The presence of leptospires was demonstrated by PCR in 13 patients before the development of antibodies, as well as in two patients who were seronegative during their illness and at autopsy. Samples from 16 patients without leptospirosis were seronegative and culture negative, and also negative by PCR. We conclude that PCR is a rapid, sensitive and specific means of diagnosing leptospiral infection, especially during the first few days of the disease.

Original languageEnglish (US)
Pages (from-to)110-114
Number of pages5
JournalJournal of Medical Microbiology
Volume43
Issue number2
StatePublished - 1995
Externally publishedYes

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Leptospirosis
Early Diagnosis
Polymerase Chain Reaction
Infection
Serology
Autopsy
Urine
Antibodies

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Brown, P. D., Gravekamp, C., Carrington, D. G., Van De Kemp, H., Hartskeerl, R. A., Edwards, C. N., ... Levett, P. N. (1995). Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. Journal of Medical Microbiology, 43(2), 110-114.

Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. / Brown, P. D.; Gravekamp, Claudia; Carrington, D. G.; Van De Kemp, H.; Hartskeerl, R. A.; Edwards, C. N.; Everard, C. O R; Terpstra, W. J.; Levett, P. N.

In: Journal of Medical Microbiology, Vol. 43, No. 2, 1995, p. 110-114.

Research output: Contribution to journalArticle

Brown, PD, Gravekamp, C, Carrington, DG, Van De Kemp, H, Hartskeerl, RA, Edwards, CN, Everard, COR, Terpstra, WJ & Levett, PN 1995, 'Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis', Journal of Medical Microbiology, vol. 43, no. 2, pp. 110-114.
Brown PD, Gravekamp C, Carrington DG, Van De Kemp H, Hartskeerl RA, Edwards CN et al. Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. Journal of Medical Microbiology. 1995;43(2):110-114.
Brown, P. D. ; Gravekamp, Claudia ; Carrington, D. G. ; Van De Kemp, H. ; Hartskeerl, R. A. ; Edwards, C. N. ; Everard, C. O R ; Terpstra, W. J. ; Levett, P. N. / Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. In: Journal of Medical Microbiology. 1995 ; Vol. 43, No. 2. pp. 110-114.
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