Evaluation of salivary DNA obtained from dental prosthesis and its applicability in forensic investigations

Isha Chauhan, G. Sugirtharaj, Sanish Sathyan, V. T. Beena, Moinak Banerjee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Aim This study evaluated the salivary DNA obtained from dental prosthesis after a period of storage and its applicability in human identification. Material and methods In first phase, DNA was extracted after a span of 1 week and 1 month from the dental prosthesis dipped in whole saliva for 15 min. It was followed by PCR and electrophoresis. In second phase, from extracted DNA samples 15 STRs (short tandem repeats) of human genomic DNA were amplified via polymerase chain reaction. Results & conclusion DNA isolated from saliva stained dental prosthesis after a period of storage and the techniques employed are adequate for further forensic analysis.

Original languageEnglish (US)
Pages (from-to)100-105
Number of pages6
JournalJournal of Forensic and Legal Medicine
Volume42
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

Keywords

  • DNA
  • Dental prosthesis
  • STRs
  • Saliva

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Law

Fingerprint

Dive into the research topics of 'Evaluation of salivary DNA obtained from dental prosthesis and its applicability in forensic investigations'. Together they form a unique fingerprint.

Cite this