TY - JOUR
T1 - Evaluation of preventive effect of Brugia malayi recombinant cystatin on mBSA-induced experimental arthritis
AU - Yadav, Ravi Shankar Prasad
AU - Khatri, Vishal
AU - Amdare, Nitin
AU - Goswami, Kalyan
AU - Shivkumar, V. B.
AU - Gangane, Nitin
AU - Reddy, Maryada Venkata Rami
N1 - Publisher Copyright:
© 2017, National Institute of Science Communication. All rights reserved.
PY - 2017/9
Y1 - 2017/9
N2 - Epidemiological and experimental studies have demonstrated the therapeutic efficacy of the helminths derived immunomodulatory molecules. In this study, we investigated the preventive effect of Brugia malayi recombinant cystatin (rBmCys) in methylated bovine serum albumin (mBSA)-induced arthritis. Mastomys coucha rats were treated with 4 doses of rBmCys (intraperitoneal) in alum adjuvant (25 µg/dose/200 µL) in intervals of 15 days. Control rats received alum only. mBSAarthritis induction was done 10 days after the last dose of rBmCys/alum. Rats were sacrificed when all the rats in mBSA group developed arthritis. Administration of rBmCys significantly (P=0.0005) protected rats from arthritis by reducing paw swelling and arthritic index. In rBmCys treated rats, histopathology of hind paw joints showed decreased synovitis, bone erosion, fibrosis and influx of inflammatory cells. This protective effect was found to be associated with significantly (P <0.005) decreased levels of IFN-γ & TNF-α and increased levels of IL-4 and IL-10 cytokines. These results have shown that prior administration of rBmCys can benefit in rheumatoid arthritis prevention.
AB - Epidemiological and experimental studies have demonstrated the therapeutic efficacy of the helminths derived immunomodulatory molecules. In this study, we investigated the preventive effect of Brugia malayi recombinant cystatin (rBmCys) in methylated bovine serum albumin (mBSA)-induced arthritis. Mastomys coucha rats were treated with 4 doses of rBmCys (intraperitoneal) in alum adjuvant (25 µg/dose/200 µL) in intervals of 15 days. Control rats received alum only. mBSAarthritis induction was done 10 days after the last dose of rBmCys/alum. Rats were sacrificed when all the rats in mBSA group developed arthritis. Administration of rBmCys significantly (P=0.0005) protected rats from arthritis by reducing paw swelling and arthritic index. In rBmCys treated rats, histopathology of hind paw joints showed decreased synovitis, bone erosion, fibrosis and influx of inflammatory cells. This protective effect was found to be associated with significantly (P <0.005) decreased levels of IFN-γ & TNF-α and increased levels of IL-4 and IL-10 cytokines. These results have shown that prior administration of rBmCys can benefit in rheumatoid arthritis prevention.
KW - Brugia malayi recombinant cystatin
KW - Rheumatoid arthritis
KW - Roundworm
KW - mBSA-induced arthritis
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M3 - Article
AN - SCOPUS:85028932289
SN - 0019-5189
VL - 55
SP - 655
EP - 660
JO - Indian Journal of Experimental Biology
JF - Indian Journal of Experimental Biology
IS - 9
ER -