Evaluation of neurobehavioral and neuroinflammatory end-points in the post-exposure period in rats sub-acutely exposed to manganese

Santos Dinamene, Batoreu M. Camila, I. Tavares de Almeida, L. Davis Randall, Mateus M. Luisa, Andrade Vanda, Ramos Ruben, Torres Edite, Aschner Michael, A. P. Marreilha dos Santos

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Manganese (Mn) can cause manganism, a neurological disorder similar to Parkinson' Disease (PD). The neurobehavioral and neuroinflammatory end-points in the Mn post exposure period have not been studied yet. Rats were injected on alternate days with 8 doses of MnCl2 (25mg/kg) or saline, then euthanized 1, 10, 30 or 70 days following the last dose. Whole-blood (WB) (p<0.05), urine (p<0.05) and brain cortical (p<0.0001) Mn levels were significantly increased 24h after the last dose. Decreases in the rats' ambulation were noted 1, 10 and 30 days after the last Mn dose (p<0.001; p<0.05; p<0.001, respectively) and also in the rearing activity at the four time-points (p<0.05). Cortical glial fibrillary acid protein immunoreactivity (GFAP-ir) was significantly increased at 1, 10, 30 (p<0.0001) and 70 (p<0.001) days after the last Mn dose, as well as tumor necrosis α (TNF-α) levels (p<0.05) but just on day 1. Taken together, the results show that, during the 70-day clearance phase of Mn, the recovery is not immediate as behavioral alterations and neuroinflammation persist long after Mn is cleared from the cortical brain compartment.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalToxicology
Volume314
Issue number1
DOIs
StatePublished - Dec 6 2013
Externally publishedYes

Keywords

  • GFAP
  • Manganese neurotoxicity
  • Neurobehavioral assays
  • Neuroinflammation
  • TNF-α

ASJC Scopus subject areas

  • Toxicology

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    Dinamene, S., Camila, B. M., Tavares de Almeida, I., Davis Randall, L., Luisa, M. M., Vanda, A., Ruben, R., Edite, T., Michael, A., & Marreilha dos Santos, A. P. (2013). Evaluation of neurobehavioral and neuroinflammatory end-points in the post-exposure period in rats sub-acutely exposed to manganese. Toxicology, 314(1), 95-99. https://doi.org/10.1016/j.tox.2013.09.008