Evaluation of a prototype real-time PCR assay for carcinogenic human papillomavirus (HPV) detection and simultaneous HPV genotype 16 (HPV16) and HPV18 genotyping

Philip E. Castle, Mark Sadorra, Tiffany Lau, Carrie Aldrich, Francisco A.R. Garcia, Janet Kornegay

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Results from a prototype real-time PCR assay that separately detected human papillomavirus genotype 16 (HPV16), HPV18, and 12 other carcinogenic HPV genotypes in aggregate (cobas 4800 HPV test) and results from a PCR assay that detects 37 HPV genotypes individually (Linear Array) were compared using a convenience sample of cervical specimens (n = 531). The percentage of total agreement between the two assays was 94.7% (95% confidence interval, 92.5 to 96.5%). The Linear Array test was more likely than cobas 4800 HPV test to test positive for the 12 other carcinogenic HPV genotypes among women without evidence of cervical disease (P = 0.004).

Original languageEnglish (US)
Pages (from-to)3344-3347
Number of pages4
JournalJournal of Clinical Microbiology
Volume47
Issue number10
DOIs
StatePublished - Oct 1 2009

ASJC Scopus subject areas

  • Microbiology (medical)

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