TY - JOUR
T1 - Evaluation of a high-intensity green fluorescent protein fluorophage method for drug- resistance diagnosis in tuberculosis for isoniazid, rifampin, and streptomycin
AU - Yu, Xia
AU - Gu, Yunting
AU - Jiang, Guanglu
AU - Ma, Yifeng
AU - Zhao, Liping
AU - Sun, Zhaogang
AU - Jain, Paras
AU - O'Donnell, Max
AU - Larsen, Michelle
AU - Jacobs, William R.
AU - Huang, Hairong
N1 - Publisher Copyright:
© 2016 Yu, Gu, Jiang, Ma, Zhao, Sun, Jain, O'Donnell, Larsen, Jacobs and Huang.
PY - 2016
Y1 - 2016
N2 - A novel method for detecting drug resistance in Mycobacterium tuberculosis using mycobacteriophage Φ2GFP10 was evaluated with clinical isolates. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well. A total of 128 clinical isolates were tested by the phage assay for isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance while conventional drug susceptibility test, by MGIT960, was used as reference. The sensitivities of Φ2GFP10 assay for INH, RIF, and STR resistance detection were 100, 98.2, and 89.3%, respectively while their specificities were 85.1, 98.6, and 95.8%, respectively. The agreement between phage and conventional assay for detecting INH, RIF, and STR resistance was 92.2, 98.4, and 93.0%, respectively. The Φ2GFP10-phage results could be available in 2 days for RIF and STR, while it takes 3 days for INH, with an estimated cost of less than $2 to test all the three antibiotics. The Φ2GFP10-phage method has the potential to be a valuable, rapid and economical screening method for detecting drug-resistant tuberculosis.
AB - A novel method for detecting drug resistance in Mycobacterium tuberculosis using mycobacteriophage Φ2GFP10 was evaluated with clinical isolates. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well. A total of 128 clinical isolates were tested by the phage assay for isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance while conventional drug susceptibility test, by MGIT960, was used as reference. The sensitivities of Φ2GFP10 assay for INH, RIF, and STR resistance detection were 100, 98.2, and 89.3%, respectively while their specificities were 85.1, 98.6, and 95.8%, respectively. The agreement between phage and conventional assay for detecting INH, RIF, and STR resistance was 92.2, 98.4, and 93.0%, respectively. The Φ2GFP10-phage results could be available in 2 days for RIF and STR, while it takes 3 days for INH, with an estimated cost of less than $2 to test all the three antibiotics. The Φ2GFP10-phage method has the potential to be a valuable, rapid and economical screening method for detecting drug-resistant tuberculosis.
KW - Diagnosis
KW - Drug resistance
KW - Mycobacteriophage
KW - Tuberculosis
KW - ΦGFP10
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U2 - 10.3389/fmicb.2016.00922
DO - 10.3389/fmicb.2016.00922
M3 - Article
AN - SCOPUS:84980021963
SN - 1664-302X
VL - 7
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - JUN
M1 - 922
ER -