Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures

Vasudev R. Rao, Eliseo A. Eugenin, Vinayaka R. Prasad

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Despite the inability of HIV-1 to infect neurons, over half of the HIV-1-infected population in the USA suffers from neurocognitive dysfunction. HIV-infected immune cells in the periphery enter the central nervous system by causing a breach in the blood-brain barrier. The damage to the neurons is mediated by viral and host toxic products released by activated and infected immune and glial cells. To evaluate the toxicity of any viral isolate, viral protein, or host inflammatory protein, we describe a protocol to assess the neuronal apoptosis and synaptic compromise in primary cultures of human neurons and astrocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages367-376
Number of pages10
Volume1354
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)10643745

Keywords

  • Chemokines
  • Cytokines
  • Gp120
  • HAD
  • HAND
  • HIV-1
  • HIV-associated dementia
  • HIV-associated neurocognitive dysfunction
  • Neuro-inflammation
  • Neuronal apoptosis
  • Neuronal damage
  • Tat

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Rao, V. R., Eugenin, E. A., & Prasad, V. R. (2016). Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures. In Methods in Molecular Biology (Vol. 1354, pp. 367-376). (Methods in Molecular Biology; Vol. 1354). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3046-3_25