Eukaryotic transcriptional dynamics: From single molecules to cell populations

Antoine Coulon, Carson C. Chow, Robert H. Singer, Daniel R. Larson

Research output: Contribution to journalReview article

154 Scopus citations

Abstract

Transcriptional regulation is achieved through combinatorial interactions between regulatory elements in the human genome and a vast range of factors that modulate the recruitment and activity of RNA polymerase. Experimental approaches for studying transcription in vivo now extend from single-molecule techniques to genome-wide measurements. Parallel to these developments is the need for testable quantitative and predictive models for understanding gene regulation. These conceptual models must also provide insight into the dynamics of transcription and the variability that is observed at the single-cell level. In this Review, we discuss recent results on transcriptional regulation and also the models those results engender. We show how a non-equilibrium description informs our view of transcription by explicitly considering time- and energy-dependence at the molecular level.

Original languageEnglish (US)
Pages (from-to)572-584
Number of pages13
JournalNature Reviews Genetics
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2013

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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