Eukaryotic cells producing ribosomes deficient in rpl1 are hypersensitive to defects in the ubiquitin-proteasome system

Kerri B. McIntosh, Arpita Bhattacharya, Ian M. Willis, Jonathan R. Warner

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

It has recently become clear that the misassembly of ribosomes in eukaryotic cells can have deleterious effects that go far beyond a simple shortage of ribosomes. In this work we find that cells deficient in ribosomal protein L1 (Rpl1; Rpl10a in mammals) produce ribosomes lacking Rpl1 that are exported to the cytoplasm and that can be incorporated into polyribosomes. The presence of such defective ribosomes leads to slow growth and appears to render the cells hypersensitive to lesions in the ubiquitin-proteasome system. Several genes that were reasonable candidates for degradation of 60S subunits lacking Rpl1 fail to do so, suggesting that key players in the surveillance of ribosomal subunits remain to be found. Interestingly, in spite of rendering the cells hypersensitive to the proteasome inhibitor MG132, shortage of Rpl1 partially suppresses the stress-invoked temporary repression of ribosome synthesis caused by MG132.

Original languageEnglish (US)
Article numbere23579
JournalPLoS One
Volume6
Issue number8
DOIs
StatePublished - 2011

Fingerprint

proteasome endopeptidase complex
Eukaryotic Cells
Proteasome Endopeptidase Complex
ubiquitin
Ubiquitin
ribosomes
Ribosomes
eukaryotic cells
Defects
Proteasome Inhibitors
Mammals
Genes
Degradation
Ribosome Subunits
polyribosomes
Polyribosomes
ribosomal proteins
rendering
cells
lesions (animal)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Eukaryotic cells producing ribosomes deficient in rpl1 are hypersensitive to defects in the ubiquitin-proteasome system. / McIntosh, Kerri B.; Bhattacharya, Arpita; Willis, Ian M.; Warner, Jonathan R.

In: PLoS One, Vol. 6, No. 8, e23579, 2011.

Research output: Contribution to journalArticle

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