Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos

Mayumi Oda, Satoshi Tanaka, Yukiko Yamazaki, Hiroshi Ohta, Misa Iwatani, Masako Suzuki, Jun Ohgane, Naka Hattori, Ryuzo Yanagimachi, Teruhiko Wakayama, Kunio Shiota

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Placental abnormalities occur frequently in cloned animals. Here, we attempted to isolate trophoblast stem (TS) cells from mouse blastocysts produced by somatic cell nuclear transfer (NT) at the blastocyst stage (NT blastocysts). Despite the predicted deficiency of the trophoblast cell lineage, we succeeded in isolating cell colonies with typical morphology of TS cells and cell lines from the NT blastocysts (ntTS cell lines) with efficiency as high as that from native blastocysts. The established 10 ntTS cell lines could be maintained in the undifferentiated state and induced to differentiate into several trophoblast subtypes in vitro. A comprehensive analysis of the transcriptional and epigenetic traits demonstrated that ntTS cells were indistinguishable from control TS cells. In addition, ntTS cells contributed exclusively to the placenta and survived until term in chimeras, indicating that ntTS cells have developmental potential as stem cells. Taken together, our data show that NT blastocysts contain cells that can produce TS cells in culture, suggesting that proper commitment to the trophoblast cell lineage in NT embryos occurs by the blastocyst stage.

Original languageEnglish (US)
Pages (from-to)16293-16297
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number38
DOIs
StatePublished - Sep 22 2009
Externally publishedYes

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Trophoblasts
Embryo Transfer
Stem Cells
Embryonic Structures
Cell Line
Blastocyst
Cell Lineage
Epigenomics
Placenta
Cell Culture Techniques

Keywords

  • Blastocyst
  • Cloned animals
  • DNA methylation
  • Epigenetics
  • Placenta

ASJC Scopus subject areas

  • General

Cite this

Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos. / Oda, Mayumi; Tanaka, Satoshi; Yamazaki, Yukiko; Ohta, Hiroshi; Iwatani, Misa; Suzuki, Masako; Ohgane, Jun; Hattori, Naka; Yanagimachi, Ryuzo; Wakayama, Teruhiko; Shiota, Kunio.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 38, 22.09.2009, p. 16293-16297.

Research output: Contribution to journalArticle

Oda, M, Tanaka, S, Yamazaki, Y, Ohta, H, Iwatani, M, Suzuki, M, Ohgane, J, Hattori, N, Yanagimachi, R, Wakayama, T & Shiota, K 2009, 'Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 38, pp. 16293-16297. https://doi.org/10.1073/pnas.0908009106
Oda, Mayumi ; Tanaka, Satoshi ; Yamazaki, Yukiko ; Ohta, Hiroshi ; Iwatani, Misa ; Suzuki, Masako ; Ohgane, Jun ; Hattori, Naka ; Yanagimachi, Ryuzo ; Wakayama, Teruhiko ; Shiota, Kunio. / Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 38. pp. 16293-16297.
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