@article{90b4c5f8aedf4898a2ba5c253bb596e8,
title = "Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids",
abstract = "Mammary stem cells (MaSCs) contribute to mammary epithelium development and homeostasis. They have been proposed as cells of origin for breast cancer. Here, we describe an organoid culture protocol for ex vivo expansion of MaSCs from mouse tissues. These organoids maintain the self-renewal of gland-reconstituting MaSCs and can be used to model tumorigenesis by introducing patient-relevant cancer drivers and mutations. Similar organoid culture can be used for long-term expansion of luminal stem/progenitor cells from normal glands and tumor-initiating cells from mammary tumors. For complete details on the use and execution of this protocol, please refer to Christin et al. (2020) and Zhang et al. (2016).",
keywords = "Cancer, Cell culture, Organoids, Stem Cells",
author = "Jihong Cui and Wenjun Guo",
note = "Funding Information: We thank the Histopathology and Analytical Imaging core facilities of Albert Einstein College of Medicine for technical assistance (supported by Einstein Cancer Center Support Grant P30 CA013330 ) and the New York State Department of Health (NYSTEM Program) for shared facility grant support ( C029154 ). This work is supported by the DOD BCRP grants W81XWH1610311 and W81XWH1810445 , the NIH/ NCI grant 1R01CA212424 , and the Mary Kay Foundation grant 04-19 (to W.G). W.G. is a V Scholar of the V Foundation for Cancer Research . Funding Information: We thank the Histopathology and Analytical Imaging core facilities of Albert Einstein College of Medicine for technical assistance (supported by Einstein Cancer Center Support Grant P30 CA013330) and the New York State Department of Health (NYSTEM Program) for shared facility grant support (C029154). This work is supported by the DOD BCRP grants W81XWH1610311 and W81XWH1810445, the NIH/NCI grant 1R01CA212424, and the Mary Kay Foundation grant 04-19 (to W.G). W.G. is a V Scholar of the V Foundation for Cancer Research. J.C. and W.G. conceived the study and wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100577",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}
