Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

Sayaka Wakayama, Martin L. Jakt, Masako Suzuki, Ryoko Araki, Takafusa Hikichi, Satoshi Kishigami, Hiroshi Ohta, Nguyen Van Thuan, Eiji Mizutani, Yuko Sakaide, Sho Senda, Satoshi Tanaka, Mitsuhiro Okada, Masashi Miyake, Masumi Abe, Shin Ichi Nishikawa, Kunio Shiota, Teruhiko Wakayama

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.

Original languageEnglish (US)
Pages (from-to)2023-2033
Number of pages11
JournalStem Cells
Volume24
Issue number9
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Blastocyst
Embryonic Stem Cells
Organism Cloning
Stem Cell Research
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Cell Line
DNA
Therapeutics

Keywords

  • Cloning
  • Embryonic stem
  • Nuclear transfer
  • Reprogramming

ASJC Scopus subject areas

  • Cell Biology

Cite this

Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts. / Wakayama, Sayaka; Jakt, Martin L.; Suzuki, Masako; Araki, Ryoko; Hikichi, Takafusa; Kishigami, Satoshi; Ohta, Hiroshi; Van Thuan, Nguyen; Mizutani, Eiji; Sakaide, Yuko; Senda, Sho; Tanaka, Satoshi; Okada, Mitsuhiro; Miyake, Masashi; Abe, Masumi; Nishikawa, Shin Ichi; Shiota, Kunio; Wakayama, Teruhiko.

In: Stem Cells, Vol. 24, No. 9, 2006, p. 2023-2033.

Research output: Contribution to journalArticle

Wakayama, S, Jakt, ML, Suzuki, M, Araki, R, Hikichi, T, Kishigami, S, Ohta, H, Van Thuan, N, Mizutani, E, Sakaide, Y, Senda, S, Tanaka, S, Okada, M, Miyake, M, Abe, M, Nishikawa, SI, Shiota, K & Wakayama, T 2006, 'Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts', Stem Cells, vol. 24, no. 9, pp. 2023-2033. https://doi.org/10.1634/stemcells.2005-0537
Wakayama, Sayaka ; Jakt, Martin L. ; Suzuki, Masako ; Araki, Ryoko ; Hikichi, Takafusa ; Kishigami, Satoshi ; Ohta, Hiroshi ; Van Thuan, Nguyen ; Mizutani, Eiji ; Sakaide, Yuko ; Senda, Sho ; Tanaka, Satoshi ; Okada, Mitsuhiro ; Miyake, Masashi ; Abe, Masumi ; Nishikawa, Shin Ichi ; Shiota, Kunio ; Wakayama, Teruhiko. / Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts. In: Stem Cells. 2006 ; Vol. 24, No. 9. pp. 2023-2033.
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