Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

Sayaka Wakayama; Martin L. Jakt; Masako Suzuki; Ryoko Araki; Takafusa Hikichi; Satoshi Kishigami; Hiroshi Ohta; Nguyen Van Thuan; Eiji Mizutani; Yuko Sakaide; Sho Senda; Satoshi Tanaka; Mitsuhiro Okada; Masashi Miyake; Masumi Abe; Shin Ichi Nishikawa; Kunio Shiota; Teruhiko Wakayama

doi:10.1634/stemcells.2005-0537

Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

Sayaka Wakayama, Martin L. Jakt, Masako Suzuki, Ryoko Araki, Takafusa Hikichi, Satoshi Kishigami, Hiroshi Ohta, Nguyen Van Thuan, Eiji Mizutani, Yuko Sakaide, Sho Senda, Satoshi Tanaka, Mitsuhiro Okada, Masashi Miyake, Masumi Abe, Shin Ichi Nishikawa, Kunio Shiota, Teruhiko Wakayama

Research output: Contribution to journal › Article › peer-review

146 Scopus citations

Abstract

Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.

Original language	English (US)
Pages (from-to)	2023-2033
Number of pages	11
Journal	STEM CELLS
Volume	24
Issue number	9
DOIs	https://doi.org/10.1634/stemcells.2005-0537
State	Published - 2006
Externally published	Yes

Keywords

Cloning
Embryonic stem
Nuclear transfer
Reprogramming

ASJC Scopus subject areas

Molecular Medicine
Developmental Biology
Cell Biology

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10.1634/stemcells.2005-0537

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Wakayama, S., Jakt, M. L., Suzuki, M., Araki, R., Hikichi, T., Kishigami, S., Ohta, H., Van Thuan, N., Mizutani, E., Sakaide, Y., Senda, S., Tanaka, S., Okada, M., Miyake, M., Abe, M., Nishikawa, S. I., Shiota, K., & Wakayama, T. (2006). Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts. STEM CELLS, 24(9), 2023-2033. https://doi.org/10.1634/stemcells.2005-0537

Wakayama, S, Jakt, ML, Suzuki, M, Araki, R, Hikichi, T, Kishigami, S, Ohta, H, Van Thuan, N, Mizutani, E, Sakaide, Y, Senda, S, Tanaka, S, Okada, M, Miyake, M, Abe, M, Nishikawa, SI, Shiota, K & Wakayama, T 2006, 'Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts', STEM CELLS, vol. 24, no. 9, pp. 2023-2033. https://doi.org/10.1634/stemcells.2005-0537

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abstract = "Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.",

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author = "Sayaka Wakayama and Jakt, {Martin L.} and Masako Suzuki and Ryoko Araki and Takafusa Hikichi and Satoshi Kishigami and Hiroshi Ohta and {Van Thuan}, Nguyen and Eiji Mizutani and Yuko Sakaide and Sho Senda and Satoshi Tanaka and Mitsuhiro Okada and Masashi Miyake and Masumi Abe and Nishikawa, {Shin Ichi} and Kunio Shiota and Teruhiko Wakayama",

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AU - Wakayama, Sayaka

AU - Jakt, Martin L.

AU - Suzuki, Masako

AU - Araki, Ryoko

AU - Hikichi, Takafusa

AU - Kishigami, Satoshi

AU - Ohta, Hiroshi

AU - Van Thuan, Nguyen

AU - Mizutani, Eiji

AU - Sakaide, Yuko

AU - Senda, Sho

AU - Tanaka, Satoshi

AU - Okada, Mitsuhiro

AU - Miyake, Masashi

AU - Abe, Masumi

AU - Nishikawa, Shin Ichi

AU - Shiota, Kunio

AU - Wakayama, Teruhiko

PY - 2006

Y1 - 2006

N2 - Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.

AB - Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.

KW - Cloning

KW - Embryonic stem

KW - Nuclear transfer

KW - Reprogramming

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JO - Stem Cells

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Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

Abstract

Keywords

ASJC Scopus subject areas

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