Epithelial to mesenchymal transition (EMT) induced by bleomycin or TFG b1/EGF in murine induced pluripotent stem cell-derived alveolar Type II-like cells

Zaida A. Alipio, Nathan Jones, Wenbin Liao, Jianchang Yang, Shilpa Kulkarni, K. Sree Kumar, Martin Hauer-Jensen, David C. Ward, Yupo Ma, Louis M. Fink

Research output: Contribution to journalArticle

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Abstract

Induced pluripotent stem (iPS) cells are derived from reprogrammed somatic cells and are similar to embryonic stem (ES) cells in morphology, gene/protein expression, and pluripotency. In this study, we explored the potential of iPS cells to differentiate into alveolar Type II (ATII)-like epithelial cells. Analysis using quantitative real time polymerase chain reaction and immunofluorescence staining showed that pulmonary surfactant proteins commonly expressed by ATII cells such as surfactant protein A (SPA), surfactant protein B (SPB), and surfactant protein C (SPC) were upregulated in the differentiated cells. Microphilopodia characteristics and lamellar bodies were observed by transmission electron microscopy and lipid deposits were verified by Nile Red and Periodic Acid Schiff staining. C3 complement protein, a specific feature of ATII cells, was present at high levels in culture supernatants demonstrating functionality of these cells in culture. These data show that the differentiated cells generated from iPS cells using a culture method developed previously (Rippon et al., 2006) are ATII-like cells.To further characterize these ATII-like cells, we tested whether they could undergo epithelial to mesenchymal transition (EMT) by exposure to drugs that induce lung fibrosis in mice, such as bleomycin, and the combination of transforming growth factor beta1 (TGF b1) and epidermal growth factor (EGF). When the ATII-like cells were exposed to either bleomycin or a TGF b1-EGF cocktail, they underwent phenotypic changes including acquisition of a mesenchymal/fibroblastic morphology, upregulation of mesenchymal markers (Col1, Vim, a-Sma, and S100A4), and downregulation of surfactant proteins and E-cadherin.We have shown that ATII-like cells can be derived from skin fibroblasts and that they respond to fibrotic stimuli. These cells provide a valuable tool for screening of agents that can potentially ameliorate or prevent diseases involving lung fibrosis.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalDifferentiation
Volume82
Issue number2
DOIs
StatePublished - Sep 2011
Externally publishedYes

Fingerprint

Induced Pluripotent Stem Cells
Epithelial-Mesenchymal Transition
Bleomycin
Epidermal Growth Factor
Surface-Active Agents
Transforming Growth Factor beta1
Fibrosis
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactant-Associated Protein A
Staining and Labeling
Periodic Acid
Cadherins
Embryonic Stem Cells
Protein C
Transmission Electron Microscopy
Lung Diseases
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Complement System Proteins
Proteins

Keywords

  • Alveolar Type II cells
  • Bleomycin
  • EMT
  • Epithelial to mesenchymal transition
  • IPS cells
  • TGF -EGF

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology
  • Cancer Research

Cite this

Epithelial to mesenchymal transition (EMT) induced by bleomycin or TFG b1/EGF in murine induced pluripotent stem cell-derived alveolar Type II-like cells. / Alipio, Zaida A.; Jones, Nathan; Liao, Wenbin; Yang, Jianchang; Kulkarni, Shilpa; Sree Kumar, K.; Hauer-Jensen, Martin; Ward, David C.; Ma, Yupo; Fink, Louis M.

In: Differentiation, Vol. 82, No. 2, 09.2011, p. 89-98.

Research output: Contribution to journalArticle

Alipio, Zaida A. ; Jones, Nathan ; Liao, Wenbin ; Yang, Jianchang ; Kulkarni, Shilpa ; Sree Kumar, K. ; Hauer-Jensen, Martin ; Ward, David C. ; Ma, Yupo ; Fink, Louis M. / Epithelial to mesenchymal transition (EMT) induced by bleomycin or TFG b1/EGF in murine induced pluripotent stem cell-derived alveolar Type II-like cells. In: Differentiation. 2011 ; Vol. 82, No. 2. pp. 89-98.
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AU - Kulkarni, Shilpa

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