Epigenetic changes in B lymphocytes associated with house dust mite allergic asthma

Marien Pascual, Masako Suzuki, Maria Isidoro-Garcia, Juana Padrón, Terrence Turner, Felix Lorente, Ignacio Dávila, John M. Greally

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Although there is no doubt about the influence of the genetic background in the onset of the allergic diseases, Epigenome-Wide Association Studies are needed to elucidate the possible relationship between allergic diseases and epigenomic dysregulation. In this study we aimed to analyze the epigenetic patterns, in terms of DNA methylation, of three well-characterized populations of house dust mite allergic subjects, aspirin-intolerant asthmatics and controls. As a first, genome-wide phase, we used the HELP assay to study the methylation patterns in CD19+ B lymphocytes in these populations, and found that there are reproducible epigenetic differences at limited numbers of loci distinguishing the groups, corroborated by bisulphite MassArray in a second validation phase of an expanded 40 subject group. These validated epigenetic changes occur at loci characterized as important for the immune response. One such locus is a new candidate gene, CYP26A1, which shows differential methylation patterns and expression levels between groups. Our results suggest that epigenomic dysregulation may contribute to the susceptibility to allergic diseases, showing for the first time differences in DNA methylation between allergic and non-allergic healthy subjects, both globally and at specific loci. These observations indicate that the epigenome may offer new pathophysiological insights and therapeutic targets in atopic diseases.

Original languageEnglish (US)
Pages (from-to)1131-1137
Number of pages7
JournalEpigenetics
Volume6
Issue number9
DOIs
StatePublished - Sep 2011

Keywords

  • Allergy
  • Aspirin intolerance
  • Asthma
  • CYP26A1
  • DNA methylation
  • Epigenetics
  • HELP assay
  • Retinoic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Fingerprint Dive into the research topics of 'Epigenetic changes in B lymphocytes associated with house dust mite allergic asthma'. Together they form a unique fingerprint.

  • Cite this

    Pascual, M., Suzuki, M., Isidoro-Garcia, M., Padrón, J., Turner, T., Lorente, F., Dávila, I., & Greally, J. M. (2011). Epigenetic changes in B lymphocytes associated with house dust mite allergic asthma. Epigenetics, 6(9), 1131-1137. https://doi.org/10.4161/epi.6.9.16061