Epidermal growth factorinduced association of the SHPTP2 protein tyrosine phosphatase with a 115-kDa phosphotyrosine protein

Keishi Yamauchi, Jeffrey E. Pessin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) stimulation of HepG2 and MH 3T3 cells expressing high levels of the human EGF receptor (3T3/ER) resulted in the tyrosine phosphorylation of a 115-kDa protein that was co-immunoprecipitated with the Src homology 2 (SH2) domain containing protein tyrosine phosphatase, SHPTP2. In contrast, activation of the EGF receptor resulted in a relatively low level (<1%) of the total SHPTP2 pool associated with the tyrosine-autophosphorylated EGF receptor itself. Similarly, quantitative immunoprecipitations also demonstrated that only trace amounts of the total EGF receptor pool were associated with SHPTP2. Further, activation of the EGF receptor did not result in any significant tyrosine phosphorylation of SHPTP2 and/or the association of the 115-kDa protein with Grb2. In comparison, activation of Jurkat cells with a T cell receptor agonist monoclonal antibody resulted in the coimmunoprecipitation of a 120-kDa tyrosine-phosphorylated protein with Grb2 and a 105-ItDa protein with SHPTP2. Thus, these data have identified the 115- and 105-kDa proteins as the predominant SHPTP2-associated phosphotyrosine proteins in EGF- and T cell receptor-activated cells, respectively.

Original languageEnglish (US)
Pages (from-to)14871-14874
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number25
StatePublished - Jun 23 1995
Externally publishedYes

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Non-Receptor Type 11 Protein Tyrosine Phosphatase
Phosphotyrosine
Protein Tyrosine Phosphatases
Epidermal Growth Factor Receptor
Association reactions
Tyrosine
Growth
Phosphorylation
Proteins
Chemical activation
T-Cell Antigen Receptor
Epidermal Growth Factor
SH2 Domain-Containing Protein Tyrosine Phosphatases
3T3 Cells
Jurkat Cells
Immunoprecipitation
Monoclonal Antibodies
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Epidermal growth factorinduced association of the SHPTP2 protein tyrosine phosphatase with a 115-kDa phosphotyrosine protein. / Yamauchi, Keishi; Pessin, Jeffrey E.

In: Journal of Biological Chemistry, Vol. 270, No. 25, 23.06.1995, p. 14871-14874.

Research output: Contribution to journalArticle

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