Epidermal growth factor receptor distribution during chemotactic responses

M. Bailly, J. Wyckoff, B. Bouzahzah, R. Hammerman, V. Sylvestre, M. Cammer, R. Pestell, Jeffrey E. Segall

Research output: Contribution to journalArticle

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Abstract

To determine the distribution of the epidermal growth factor (EGF) receptor (EGFR) on the surface of cells responding to EGF as a chemoattractant, an EGFR-green fluorescent protein chimera was expressed in the MTLn3 mammary carcinoma cell line. The chimera was functional and easily visualized on the cell surface. In contrast to other studies indicating that the EGFR might be localized to certain regions of the plasma membrane, we found that the chimera is homogeneously distributed on the plasma membrane and becomes most concentrated in vesicles after endocytosis. In spatial gradients of EGF, endocytosed receptor accumulates on the upgradient side of the cell. Visualization of the binding of fluorescent EGF to cells reveals that the affinity properties of the receptor, together with its expression level on cells, can provide an initial amplification step in spatial gradient sensing.

Original languageEnglish (US)
Pages (from-to)3873-3883
Number of pages11
JournalMolecular Biology of the Cell
Volume11
Issue number11
StatePublished - 2000

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Epidermal Growth Factor Receptor
Endocytosis
Epidermal Growth Factor
Cell Membrane
Chemotactic Factors
Green Fluorescent Proteins
Breast Neoplasms
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Bailly, M., Wyckoff, J., Bouzahzah, B., Hammerman, R., Sylvestre, V., Cammer, M., ... Segall, J. E. (2000). Epidermal growth factor receptor distribution during chemotactic responses. Molecular Biology of the Cell, 11(11), 3873-3883.

Epidermal growth factor receptor distribution during chemotactic responses. / Bailly, M.; Wyckoff, J.; Bouzahzah, B.; Hammerman, R.; Sylvestre, V.; Cammer, M.; Pestell, R.; Segall, Jeffrey E.

In: Molecular Biology of the Cell, Vol. 11, No. 11, 2000, p. 3873-3883.

Research output: Contribution to journalArticle

Bailly, M, Wyckoff, J, Bouzahzah, B, Hammerman, R, Sylvestre, V, Cammer, M, Pestell, R & Segall, JE 2000, 'Epidermal growth factor receptor distribution during chemotactic responses', Molecular Biology of the Cell, vol. 11, no. 11, pp. 3873-3883.
Bailly M, Wyckoff J, Bouzahzah B, Hammerman R, Sylvestre V, Cammer M et al. Epidermal growth factor receptor distribution during chemotactic responses. Molecular Biology of the Cell. 2000;11(11):3873-3883.
Bailly, M. ; Wyckoff, J. ; Bouzahzah, B. ; Hammerman, R. ; Sylvestre, V. ; Cammer, M. ; Pestell, R. ; Segall, Jeffrey E. / Epidermal growth factor receptor distribution during chemotactic responses. In: Molecular Biology of the Cell. 2000 ; Vol. 11, No. 11. pp. 3873-3883.
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