TY - JOUR
T1 - Epidermal growth factor receptor distribution during chemotactic responses
AU - Bailly, M.
AU - Wyckoff, J.
AU - Bouzahzah, B.
AU - Hammerman, R.
AU - Sylvestre, V.
AU - Cammer, M.
AU - Pestell, R.
AU - Segall, J. E.
PY - 2000
Y1 - 2000
N2 - To determine the distribution of the epidermal growth factor (EGF) receptor (EGFR) on the surface of cells responding to EGF as a chemoattractant, an EGFR-green fluorescent protein chimera was expressed in the MTLn3 mammary carcinoma cell line. The chimera was functional and easily visualized on the cell surface. In contrast to other studies indicating that the EGFR might be localized to certain regions of the plasma membrane, we found that the chimera is homogeneously distributed on the plasma membrane and becomes most concentrated in vesicles after endocytosis. In spatial gradients of EGF, endocytosed receptor accumulates on the upgradient side of the cell. Visualization of the binding of fluorescent EGF to cells reveals that the affinity properties of the receptor, together with its expression level on cells, can provide an initial amplification step in spatial gradient sensing.
AB - To determine the distribution of the epidermal growth factor (EGF) receptor (EGFR) on the surface of cells responding to EGF as a chemoattractant, an EGFR-green fluorescent protein chimera was expressed in the MTLn3 mammary carcinoma cell line. The chimera was functional and easily visualized on the cell surface. In contrast to other studies indicating that the EGFR might be localized to certain regions of the plasma membrane, we found that the chimera is homogeneously distributed on the plasma membrane and becomes most concentrated in vesicles after endocytosis. In spatial gradients of EGF, endocytosed receptor accumulates on the upgradient side of the cell. Visualization of the binding of fluorescent EGF to cells reveals that the affinity properties of the receptor, together with its expression level on cells, can provide an initial amplification step in spatial gradient sensing.
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U2 - 10.1091/mbc.11.11.3873
DO - 10.1091/mbc.11.11.3873
M3 - Article
C2 - 11071913
AN - SCOPUS:0033729987
SN - 1059-1524
VL - 11
SP - 3873
EP - 3883
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 11
ER -