TY - JOUR
T1 - Ephrin-B1 and ephrin-B2 mediate EphB-dependent presynaptic development via syntenin-1
AU - McClelland, Andrew C.
AU - Sheffler-Collins, Sean I.
AU - Kayser, Matthew S.
AU - Dalva, Matthew B.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - The development of central nervous system synapses requires precise coordination between presynaptic and postsynaptic components. The EphB family controls postsynaptic development by interacting with glutamate receptors and regulating dendritic filopodia motility, but how EphBs induce the formation of presynaptic specializations is less well understood. Here, we show that knockdown of presynaptic ephrin-B1, ephrin-B2, or syntenin-1, but not ephrin-B3, prevents EphB-dependent presynaptic development. Ephrin-B1, ephrin-B2, and syntenin-1 are clustered together with presynaptic markers, suggesting that these molecules function jointly in presynaptic development. Knockdown of ephrin-B1 or ephrin-B2 reduces the number of synaptic specializations and the colocalization of syntenin-1 with synaptic markers. Simultaneous knockdown of ephrin-B1 and ephrin-B2 suggests that they function independently in the formation of synaptic contacts, but act together to recruit syntenin-1 to presynaptic terminals. Taken together, these results demonstrate that ephrin-B1 and ephrin-B2 function with EphB to mediate presynaptic development via syntenin-1.
AB - The development of central nervous system synapses requires precise coordination between presynaptic and postsynaptic components. The EphB family controls postsynaptic development by interacting with glutamate receptors and regulating dendritic filopodia motility, but how EphBs induce the formation of presynaptic specializations is less well understood. Here, we show that knockdown of presynaptic ephrin-B1, ephrin-B2, or syntenin-1, but not ephrin-B3, prevents EphB-dependent presynaptic development. Ephrin-B1, ephrin-B2, and syntenin-1 are clustered together with presynaptic markers, suggesting that these molecules function jointly in presynaptic development. Knockdown of ephrin-B1 or ephrin-B2 reduces the number of synaptic specializations and the colocalization of syntenin-1 with synaptic markers. Simultaneous knockdown of ephrin-B1 and ephrin-B2 suggests that they function independently in the formation of synaptic contacts, but act together to recruit syntenin-1 to presynaptic terminals. Taken together, these results demonstrate that ephrin-B1 and ephrin-B2 function with EphB to mediate presynaptic development via syntenin-1.
KW - Coculture
KW - Cortex
KW - Eph
KW - Excitatory synapse
KW - Synaptogenesis
UR - http://www.scopus.com/inward/record.url?scp=73949092350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=73949092350&partnerID=8YFLogxK
U2 - 10.1073/pnas.0811862106
DO - 10.1073/pnas.0811862106
M3 - Article
C2 - 19915143
AN - SCOPUS:73949092350
SN - 0027-8424
VL - 106
SP - 20487
EP - 20492
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 48
ER -