Abstract
N-ribohydrolases and transferases act on nucleosides, nucleotides and oligonucleotides to effect base removal. Advances in mechanistic and structural analysis have established that enzymes of N-riboside scission act by combinations of leaving-group and ribosyl activation. Alternative O-riboside substrates have been developed for mechanistic diagnosis. Transition-state structures have been determined, and powerful inhibitors have been designed from structural and transition-state information.
Original language | English (US) |
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Pages (from-to) | 323-331 |
Number of pages | 9 |
Journal | Current Opinion in Chemical Biology |
Volume | 1 |
Issue number | 3 |
DOIs | |
State | Published - Oct 1997 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry