Entry of hepatitis C virus and human immunodeficiency virus is selectively inhibited by carbohydrate-binding agents but not by polyanions

Claire Bertaux, Dirk Daelemans, Laurent Meertens, Emmanuel G. Cormier, John F. Reinus, Willy J. Peumans, Els J M Van Damme, Yasuhiro Igarashi, Toshikazu Oki, Dominique Schols, Tatjana Dragic, Jan Balzarini

Research output: Contribution to journalArticle

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Abstract

We studied the antiviral activity of carbohydrate-binding agents (CBAs), including several plant lectins and the non-peptidic small-molecular-weight antibiotic pradimicin A (PRM-A). These agents efficiently prevented hepatitis C virus (HCV) and human immunodeficiency virus type 1 (HIV-1) infection of target cells by inhibiting the viral entry. CBAs were also shown to prevent HIV and HCV capture by DC-SIGN-expressing cells. Surprisingly, infection by other enveloped viruses such as herpes simplex viruses, respiratory syncytial virus and parainfluenza-3 virus was not inhibited by these agents pointing to a high degree of specificity. Mannan reversed the antiviral activity of CBAs, confirming their association with viral envelope-associated glycans. In contrast, polyanions such as dextran sulfate-5000 and sulfated polyvinylalcohol inhibited HIV entry but were devoid of any activity against HCV infection, indicating that they act through a different mechanism. CBAs could be considered as prime drug leads for the treatment of chronic viral infections such as HCV by preventing viral entry into target cells. They may represent an attractive new option for therapy of HCV/HIV coinfections. CBAs may also have the potential to prevent HCV/HIV transmission.

Original languageEnglish (US)
Pages (from-to)40-50
Number of pages11
JournalVirology
Volume366
Issue number1
DOIs
StatePublished - Sep 15 2007

Fingerprint

Hepacivirus
Carbohydrates
HIV
Virus Diseases
Antiviral Agents
Plant Lectins
Viruses
Mannans
Paramyxoviridae Infections
Dextran Sulfate
Respiratory Syncytial Viruses
Simplexvirus
Coinfection
Polysaccharides
polyanions
HIV-1
Molecular Weight
Anti-Bacterial Agents
Infection
Pharmaceutical Preparations

Keywords

  • Carbohydrate-binding agents (CBA)
  • DC-SIGN
  • HCV
  • HIV
  • Lectins
  • Transmission

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Entry of hepatitis C virus and human immunodeficiency virus is selectively inhibited by carbohydrate-binding agents but not by polyanions. / Bertaux, Claire; Daelemans, Dirk; Meertens, Laurent; Cormier, Emmanuel G.; Reinus, John F.; Peumans, Willy J.; Van Damme, Els J M; Igarashi, Yasuhiro; Oki, Toshikazu; Schols, Dominique; Dragic, Tatjana; Balzarini, Jan.

In: Virology, Vol. 366, No. 1, 15.09.2007, p. 40-50.

Research output: Contribution to journalArticle

Bertaux, C, Daelemans, D, Meertens, L, Cormier, EG, Reinus, JF, Peumans, WJ, Van Damme, EJM, Igarashi, Y, Oki, T, Schols, D, Dragic, T & Balzarini, J 2007, 'Entry of hepatitis C virus and human immunodeficiency virus is selectively inhibited by carbohydrate-binding agents but not by polyanions', Virology, vol. 366, no. 1, pp. 40-50. https://doi.org/10.1016/j.virol.2007.04.008
Bertaux, Claire ; Daelemans, Dirk ; Meertens, Laurent ; Cormier, Emmanuel G. ; Reinus, John F. ; Peumans, Willy J. ; Van Damme, Els J M ; Igarashi, Yasuhiro ; Oki, Toshikazu ; Schols, Dominique ; Dragic, Tatjana ; Balzarini, Jan. / Entry of hepatitis C virus and human immunodeficiency virus is selectively inhibited by carbohydrate-binding agents but not by polyanions. In: Virology. 2007 ; Vol. 366, No. 1. pp. 40-50.
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