Enteral intestinal alkaline phosphatase administration in newborns decreases iNOS expression in a neonatal necrotizing enterocolitis rat model

Rebecca M. Rentea, Jennifer L. Liedel, Katherine Fredrich, Kirkwood Pritchard, Keith T. Oldham, Pippa M. Simpson, David M. Gourlay

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Purpose: To determine if intestinal alkaline phosphatase (IAP) decreases intestinal injury resulting from experimentally induced necrotizing enterocolitis (NEC). We hypothesized that IAP administration prevents the initial development of NEC related intestinal inflammation. Methods: Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on day 1 of life. Pre-term pups were exposed to intermittent hypoxia and formula containing LPS to induce NEC. Select NEC pups were given 40, 4 or 0.4 units/kg of bovine IAP (NEC + IAP40u, IAP4u or IAP0.4u) enterally, once daily. Ileal sections were evaluated by real-time PCR (qRT-PCR) for IAP, iNOS, IL-1β, IL-6, and TNF-α mRNA and immunofluorescence for 3-nitrotyrosine (3-NT). Results: Experimentally induced NEC decreased IAP mRNA expression by 66% (p ≤ 0.001). IAP supplementation increased IAP mRNA expression to control. Supplemental enteral IAP decreased nitrosative stress as measured by iNOS mRNA expression and 3-NT staining in the NEC stressed pups (p ≤ 0.01), as well as decreased intestinal TNF-α mRNA expression. In addition, IAP decreased LSP translocation into the serum in the treated pups. Conclusions: We conclude that enterally administered IAP prevents NEC-related intestinal injury and inflammation. Enteral IAP may prove a useful strategy in the prevention of NEC in preterm neonates.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalJournal of Pediatric Surgery
Volume48
Issue number1
DOIs
StatePublished - Jan 1 2013

Keywords

  • Formula
  • Inflammation
  • Intestinal alkaline phosphatase
  • Necrotizing enterocolitis
  • Prematurity

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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