Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity

Mai Jing Liao, Cheng Zhang Cheng, Beiyan Zhou, Drazen B. Zimonjic, Sendurai A. Mani, Megan Kaba, Ann Gifford, Ferenc Reinhardt, Nicholas C. Popescu, Wenjun Guo, Ng Eaton Elinor, Harvey F. Lodish, Robert A. Weinberg

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The identification of mammary gland stem cells (MGSC) or progenitors is important for the study of normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-forming cells after prolonged culture, which provided preliminary insight into the genomic stability of these cells. Our identification of newcell surface markers for enriching mammosphere-initiating cells, including endoglin and prion protein, will facilitate the elucidation of the cell biology of MGSC.

Original languageEnglish (US)
Pages (from-to)8131-8138
Number of pages8
JournalCancer Research
Volume67
Issue number17
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

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Human Mammary Glands
Population
Breast
Stem Cells
Genomic Instability
Cytogenetic Analysis
Cell Biology
Adipose Tissue
Carcinogenesis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Liao, M. J., Cheng, C. Z., Zhou, B., Zimonjic, D. B., Mani, S. A., Kaba, M., ... Weinberg, R. A. (2007). Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity. Cancer Research, 67(17), 8131-8138. https://doi.org/10.1158/0008-5472.CAN-06-4493

Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity. / Liao, Mai Jing; Cheng, Cheng Zhang; Zhou, Beiyan; Zimonjic, Drazen B.; Mani, Sendurai A.; Kaba, Megan; Gifford, Ann; Reinhardt, Ferenc; Popescu, Nicholas C.; Guo, Wenjun; Elinor, Ng Eaton; Lodish, Harvey F.; Weinberg, Robert A.

In: Cancer Research, Vol. 67, No. 17, 01.09.2007, p. 8131-8138.

Research output: Contribution to journalArticle

Liao, MJ, Cheng, CZ, Zhou, B, Zimonjic, DB, Mani, SA, Kaba, M, Gifford, A, Reinhardt, F, Popescu, NC, Guo, W, Elinor, NE, Lodish, HF & Weinberg, RA 2007, 'Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity', Cancer Research, vol. 67, no. 17, pp. 8131-8138. https://doi.org/10.1158/0008-5472.CAN-06-4493
Liao, Mai Jing ; Cheng, Cheng Zhang ; Zhou, Beiyan ; Zimonjic, Drazen B. ; Mani, Sendurai A. ; Kaba, Megan ; Gifford, Ann ; Reinhardt, Ferenc ; Popescu, Nicholas C. ; Guo, Wenjun ; Elinor, Ng Eaton ; Lodish, Harvey F. ; Weinberg, Robert A. / Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity. In: Cancer Research. 2007 ; Vol. 67, No. 17. pp. 8131-8138.
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