Abstract
The identification of mammary gland stem cells (MGSC) or progenitors is important for the study of normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-forming cells after prolonged culture, which provided preliminary insight into the genomic stability of these cells. Our identification of newcell surface markers for enriching mammosphere-initiating cells, including endoglin and prion protein, will facilitate the elucidation of the cell biology of MGSC.
Original language | English (US) |
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Pages (from-to) | 8131-8138 |
Number of pages | 8 |
Journal | Cancer research |
Volume | 67 |
Issue number | 17 |
DOIs | |
State | Published - Sep 1 2007 |
Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research