Enhanced platelet adherence and aggregation in Chagas' disease

A potential pathogenic mechanism for cardiomyopathy

H. B. Tanowitz, Edward R. Burns, A. K. Sinha, N. N. Kahn, S. A. Morris, S. M. Factor, Victor Bernard Hatcher, J. P. Bilezikian, Stephen G. Baum, M. Wittner

Research output: Contribution to journalArticle

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Abstract

Spasm and thrombosis of the coronary microcirculation has been implicated in the pathogenesis of the cardiomyopathy of Chagas' disease. We demonstrate that increases in platelet adherence and aggregation accompany Trypanosoma cruzi infection and may contribute to the observed microvascular pathology. Scanning electron microscopy and radiolabeled platelet studies revealed that platelet adherence to T. cruzi-infected human endothelial cells was significantly increased when compared to controls (P = 0.024). In in vitro experiments, we determined the influence of infection on prostacyclin production, a marker of endothelial cell perturbation. The basal levels of 6-keto-prostaglandin F(1α) was significantly greater in the supernatant of infected endothelial cells than in those of uninfected endothelial cells (P < 0.05). The influence of infection was assessed on platelet aggregation at days 5 and 12 post-infection in A/J mice. Platelets from T. cruzi-infected mice were 2-6-fold more sensitive to aggregation induced by adenosine diphosphate and sodium arachidonate than controls. Thromboxane B2 levels in the plasma of infected mice were greater than controls. These data support the hypothesis that heightened platelet reactivity and endothelial cell dysfunction are associated with acute Chagas' disease and may cause coronary microvascular spasm and/or occlusion.

Original languageEnglish (US)
Pages (from-to)274-281
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume43
Issue number3
StatePublished - 1990

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Chagas Disease
Cardiomyopathies
Platelet Aggregation
Endothelial Cells
Trypanosoma cruzi
Blood Platelets
Spasm
Infection
Coronary Thrombosis
Thromboxane B2
Prostaglandins F
Acute Disease
Epoprostenol
Microcirculation
Arachidonic Acid
Electron Scanning Microscopy
Adenosine Diphosphate
Pathology

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Enhanced platelet adherence and aggregation in Chagas' disease : A potential pathogenic mechanism for cardiomyopathy. / Tanowitz, H. B.; Burns, Edward R.; Sinha, A. K.; Kahn, N. N.; Morris, S. A.; Factor, S. M.; Hatcher, Victor Bernard; Bilezikian, J. P.; Baum, Stephen G.; Wittner, M.

In: American Journal of Tropical Medicine and Hygiene, Vol. 43, No. 3, 1990, p. 274-281.

Research output: Contribution to journalArticle

Tanowitz, H. B. ; Burns, Edward R. ; Sinha, A. K. ; Kahn, N. N. ; Morris, S. A. ; Factor, S. M. ; Hatcher, Victor Bernard ; Bilezikian, J. P. ; Baum, Stephen G. ; Wittner, M. / Enhanced platelet adherence and aggregation in Chagas' disease : A potential pathogenic mechanism for cardiomyopathy. In: American Journal of Tropical Medicine and Hygiene. 1990 ; Vol. 43, No. 3. pp. 274-281.
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