Enhanced glucose transport, but not phosphorylation capacity, ameliorates lipopolysaccharide-induced impairments in insulin-stimulated muscle glucose uptake

Yolanda F. Otero, Kimberly X. Mulligan, Tammy M. Barnes, Eric A. Ford, Carlo M. Malabanan, Haihong Zong, Jeffrey E. Pessin, David H. Wasserman, Owen P. McGuinness

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Lipopolysaccharide (LPS) is known to impair insulin-stimulated muscle glucose uptake (MGU). We determined if increased glucose transport (GLUT4) or phosphorylation capacity (hexokinase II; HKII) could overcome the impairment in MGU. We used mice that overexpressed GLUT4 (GLUT4Tg) or HKII (HKTg) in skeletal muscle. Studies were performed in conscious, chronically catheterized (carotid artery and jugular vein) mice. Mice received an intravenous bolus of either LPS (10mg/g body weight) or vehicle (VEH). After 5 h, a hyperinsulinemic-euglycemic clamp was performed. As MGU is also dependent on cardiovascular function that is negatively affected by LPS, cardiac function was assessed using echocardiography. LPS decreased whole body glucose disposal and MGU in wild-type (WT) and HKTg mice. In contrast, the decrease was attenuated in GLUT4Tg mice. Although membrane-associated GLUT4 was increased in VEH-treated GLUT4Tg mice, LPS impaired membrane-associated GLUT4 in GLUT4Tg mice to the same level as LPS-treated WTmice. This suggested that overexpression of GLUT4 had further benefits beyond preserving transport activity. In fact, GLUT4 overexpression attenuated the LPS-induced decrease in cardiac function. The maintenance of MGU in GLUT4Tg mice following LPS was accompanied by sustained anaerobic glycolytic flux as suggested by increased muscle Pdk4 expression, and elevated lactate availability. Thus, enhanced glucose transport, but not phosphorylation capacity, ameliorates LPSinduced impairments inMGU. This benefit is mediated by long-termadaptations to the overexpression of GLUT4 that sustain muscle anaerobic glycolytic flux and cardiac function in response to LPS.

Original languageEnglish (US)
Pages (from-to)677-685
Number of pages9
JournalShock
Volume45
Issue number6
DOIs
StatePublished - Jun 1 2016

Keywords

  • Cardiac output
  • Endotoxin
  • Inflammation
  • Insulin resistance

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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