Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels

Cameron L. Nemeth, Kajohnkiart Janebodin, Alex E. Yuan, James E. Dennis, Morayma Reyes Gil, Deok Ho Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have examined the effects of surface nanotopography and hyaluronic acid (HA) on in vitro chondrogenesis of dental pulp stem cells (DPSCs). Ultraviolet-assisted capillary force lithography was employed to fabricate well-defined nanostructured scaffolds of composite PEG-GelMA-HA hydrogels that consist of poly(ethylene glycol) dimethacrylate (PEGDMA), methacrylated gelatin (GelMA), and HA. Using this microengineered platform, we first demonstrated that DPSCs formed three-dimensional spheroids, which provide an appropriate environment for in vitro chondrogenic differentiation. We also found that DPSCs cultured on nanopatterned PEG-GelMA-HA scaffolds showed a significant upregulation of the chondrogenic gene markers (Sox9, Alkaline phosphatase, Aggrecan, Procollagen type II, and Procollagen type X), while downregulating the pluripotent stem cell gene, Nanog, and epithelial-mesenchymal genes (Twist, Snail, Slug) compared with tissue culture polystyrene-cultured DPSCs. Immunocytochemistry showed more extensive deposition of collagen type II in DPSCs cultured on the nanopatterned PEG-GelMA-HA scaffolds. These findings suggest that nanotopography and HA provide important cues for promoting chondrogenic differentiation of DPSCs.

Original languageEnglish (US)
Pages (from-to)2817-2829
Number of pages13
JournalTissue Engineering - Part A.
Volume20
Issue number21-22
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

Fingerprint

Hyaluronic acid
Dental Pulp
Hydrogels
Hyaluronic Acid
Stem cells
Polyethylene glycols
Pulp
Stem Cells
Scaffolds
Collagen Type II
Genes
Collagen Type X
Aggrecans
Chondrogenesis
Pluripotent Stem Cells
Gastropoda
Polystyrenes
Tissue culture
Gelatin
Phosphatases

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels. / Nemeth, Cameron L.; Janebodin, Kajohnkiart; Yuan, Alex E.; Dennis, James E.; Reyes Gil, Morayma; Kim, Deok Ho.

In: Tissue Engineering - Part A., Vol. 20, No. 21-22, 01.11.2014, p. 2817-2829.

Research output: Contribution to journalArticle

Nemeth, Cameron L. ; Janebodin, Kajohnkiart ; Yuan, Alex E. ; Dennis, James E. ; Reyes Gil, Morayma ; Kim, Deok Ho. / Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels. In: Tissue Engineering - Part A. 2014 ; Vol. 20, No. 21-22. pp. 2817-2829.
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