Endothelial monocyte activating polypeptide-II induced gene expression changes in endothelial cells

Anita T. Tandle, Chiara Mazzanti, H. Richard Alexander, David D. Roberts, Steven K. Libutti

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

In the current study we used microarray (MA) analysis to examine gene expression changes in human umbilical vein endothelial cells (HUVEC) exposed to the tumor-derived cytokine, endothelial monocyte-activating polypeptide-II (EMAP-II). HUVEC treated with EMAP-II for 0.5, 1, 2, 4 and 8 h, were analyzed using 10K cDNA arrays. Our results demonstrated that changes in gene expression of <0.5 and >2 fold were seen for 69 genes and the majority of gene changes occurred early. Validation of MA analysis for 10 genes by real time RT-PCR, demonstrated the gene changes to be consistent and specific to HUVEC when compared to human fibroblasts treated with EMAP-II. Among these genes, downregulated in ovarian cancer 1 (DOC1) gene was studied further because of its possible role in EMAP-II induced cytoskeletal remodeling. DOC1 expression was silenced using small interfering RNA. SiRNA to DOC1 completely abolished EMAP-II stimulated gene expression of DOC1. Silencing of DOC1 gene expression reversed the modulatory effect of EMAP-II on 4 other genes, suggesting that DOC1 might play a role in mediating some of the effects of EMAP-II on endothelial cells.

Original languageEnglish (US)
Pages (from-to)347-358
Number of pages12
JournalCytokine
Volume30
Issue number6
DOIs
StatePublished - Jun 21 2005

Keywords

  • DOC1
  • EMAP-II
  • Endothelial cells
  • Microarray analysis
  • SiRNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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