Endothelial cell-derived high molecular weight von Willebrand factor is converted into the plasma multimer pattern by granulocyte proteases

Han Mou Tsai, Ronald L. Nagel, Victor Bernard Hatcher, Ira I. Sussman

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have previously found that the von Willebrand factor released by cultured human umbilical vein endothelial cells appeared as a single high molecular weight band in glyoxyl agarose electrophoresis. In the present studies we report that this high molecular weight endothelial cell-derived von Willebrand factor, when incubated with granulocyte lysates, was cleaved into a series of multimers indistinguishable from those seen in normal plasma (or type II von Willebrand disease). This von Willebrand factor-cleaving activity was released from granulocytes by calcium ionophore A23187 but was not detected in cytosolic fractions depleted of granular contents. It was inhibited by the serine protease inhibitor phenylmethylsulfonyl fluoride. This von Willebrand factor-cleaving activity thus provides a possible mechanism for the generation of plasma von Willebrand factor multimers from the high molecular weight form of von Willebrand factor secreted by endothelial cells.

Original languageEnglish (US)
Pages (from-to)980-985
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume158
Issue number3
DOIs
StatePublished - Feb 15 1989

Fingerprint

Endothelial cells
von Willebrand Factor
Granulocytes
Peptide Hydrolases
Endothelial Cells
Molecular Weight
Molecular weight
Plasmas
Type 2 von Willebrand Disease
Phenylmethylsulfonyl Fluoride
Serine Proteinase Inhibitors
Calcium Ionophores
Human Umbilical Vein Endothelial Cells
Calcimycin
Electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Endothelial cell-derived high molecular weight von Willebrand factor is converted into the plasma multimer pattern by granulocyte proteases. / Tsai, Han Mou; Nagel, Ronald L.; Hatcher, Victor Bernard; Sussman, Ira I.

In: Biochemical and Biophysical Research Communications, Vol. 158, No. 3, 15.02.1989, p. 980-985.

Research output: Contribution to journalArticle

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