Endogenous formation and repair of oxidatively induced G[8-5 m]T intrastrand cross-link lesion

Jin Wang, Huachuan Cao, Changjun You, Bifeng Yuan, Ralf Bahde, Sanjeev Gupta, Chikako Nishigori, Laura J. Niedernhofer, Philip J. Brooks, Yinsheng Wang

Research output: Contribution to journalArticle

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Abstract

SExposure to reactive oxygen species (ROS) can give rise to the formation of various DNA damage products. Among them, d(G[8-5m]T) can be induced in isolated DNA treated with Fenton reagents and in cultured human cells exposed to c-rays, d(G[8-5m]T) can be recognized and incised by purified Escherichia coli UvrABC nuclease. However, it remains unexplored whether d(G[8-5 m]T) accumulates in mammalian tissues and whether it is a substrate for nucleotide excision repair (NER) in vivo. Here, we found that d(G[8-5 m]T) could be detected in DNA isolated from tissues of healthy humans and animals, and elevated endogenous ROS generation enhanced the accumulation of this lesion in tissues of a rat model of Wilson's disease. Additionally, XPA-deficient human brain and mouse liver as well as various types of tissues of ERCC1-deficient mice contained higher levels of d(G[8-5 m]T) but not ROS-induced single-nucleobase lesions than the corresponding normal controls. Together, our studies established that d(G[8-5 m]T) can be induced endogenously in mammalian tissues and constitutes a substrate for NER in vivo.

Original languageEnglish (US)
Pages (from-to)7368-7374
Number of pages7
JournalNucleic Acids Research
Volume40
Issue number15
DOIs
StatePublished - Aug 2012

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Reactive Oxygen Species
DNA Repair
Hepatolenticular Degeneration
DNA
DNA Damage
Cultured Cells
Escherichia coli
Liver
Brain
G(8-5m)T
Fenton's reagent

ASJC Scopus subject areas

  • Genetics

Cite this

Endogenous formation and repair of oxidatively induced G[8-5 m]T intrastrand cross-link lesion. / Wang, Jin; Cao, Huachuan; You, Changjun; Yuan, Bifeng; Bahde, Ralf; Gupta, Sanjeev; Nishigori, Chikako; Niedernhofer, Laura J.; Brooks, Philip J.; Wang, Yinsheng.

In: Nucleic Acids Research, Vol. 40, No. 15, 08.2012, p. 7368-7374.

Research output: Contribution to journalArticle

Wang, J, Cao, H, You, C, Yuan, B, Bahde, R, Gupta, S, Nishigori, C, Niedernhofer, LJ, Brooks, PJ & Wang, Y 2012, 'Endogenous formation and repair of oxidatively induced G[8-5 m]T intrastrand cross-link lesion', Nucleic Acids Research, vol. 40, no. 15, pp. 7368-7374. https://doi.org/10.1093/nar/gks357
Wang, Jin ; Cao, Huachuan ; You, Changjun ; Yuan, Bifeng ; Bahde, Ralf ; Gupta, Sanjeev ; Nishigori, Chikako ; Niedernhofer, Laura J. ; Brooks, Philip J. ; Wang, Yinsheng. / Endogenous formation and repair of oxidatively induced G[8-5 m]T intrastrand cross-link lesion. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 15. pp. 7368-7374.
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