Endocytosis by random initiation and stabilization of clathrin-coated pits

Marcelo Ehrlich, Werner Boll, Antoine Van Oijen, Ramesh Hariharan, Kartik Chandran, Max L. Nibert, Tomas Kirchhausen

Research output: Contribution to journalArticle

579 Citations (Scopus)

Abstract

Clathrin-coated vesicles carry traffic from the plasma membrane to endosomes. We report here the real-time visualization of cargo sorting and endocytosis by clathrin-coated pits in living cells. We have detected the formation of coats by monitoring incorporation of fluorescently tagged clathrin or its adaptor AP-2; we have also followed clathrin-mediated uptake of transferrin and of single LDL or reovirus particles. The intensity of a cargo-loaded clathrin cluster grows steadily during its lifetime, and the time required to complete assembly is proportional to the size of the cargo particle. These results are consistent with a nucleation-growth mechanism and an approximately constant growth rate. There are no strongly preferred nucleation sites. A proportion of the nucleation events are weak and short lived. Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture.

Original languageEnglish (US)
Pages (from-to)591-605
Number of pages15
JournalCell
Volume118
Issue number5
DOIs
StatePublished - Sep 3 2004
Externally publishedYes

Fingerprint

Clathrin
Endocytosis
Stabilization
Nucleation
Clathrin-Coated Vesicles
Endosomes
Transferrin
Growth
Particle Size
Cell membranes
Sorting
Cell Membrane
Visualization
Cells
Monitoring

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Ehrlich, M., Boll, W., Van Oijen, A., Hariharan, R., Chandran, K., Nibert, M. L., & Kirchhausen, T. (2004). Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell, 118(5), 591-605. https://doi.org/10.1016/j.cell.2004.08.017

Endocytosis by random initiation and stabilization of clathrin-coated pits. / Ehrlich, Marcelo; Boll, Werner; Van Oijen, Antoine; Hariharan, Ramesh; Chandran, Kartik; Nibert, Max L.; Kirchhausen, Tomas.

In: Cell, Vol. 118, No. 5, 03.09.2004, p. 591-605.

Research output: Contribution to journalArticle

Ehrlich, M, Boll, W, Van Oijen, A, Hariharan, R, Chandran, K, Nibert, ML & Kirchhausen, T 2004, 'Endocytosis by random initiation and stabilization of clathrin-coated pits', Cell, vol. 118, no. 5, pp. 591-605. https://doi.org/10.1016/j.cell.2004.08.017
Ehrlich M, Boll W, Van Oijen A, Hariharan R, Chandran K, Nibert ML et al. Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell. 2004 Sep 3;118(5):591-605. https://doi.org/10.1016/j.cell.2004.08.017
Ehrlich, Marcelo ; Boll, Werner ; Van Oijen, Antoine ; Hariharan, Ramesh ; Chandran, Kartik ; Nibert, Max L. ; Kirchhausen, Tomas. / Endocytosis by random initiation and stabilization of clathrin-coated pits. In: Cell. 2004 ; Vol. 118, No. 5. pp. 591-605.
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