Emulsion based selection of T7 promoters of varying activity

Eric A. Davidson, Thomas Van Blarcom, Matthew Levy, Andrew D. Ellington

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

The ability to build and control complex biological systems is greatly enhanced by the generation of related parts with varying strengths. In this way, various parts can be strung together and the connectivity and expression levels can be matched for the desired system performance. Engineered gene circuits, both in vivo and in vitro, often utilize the T7 RNA polymerase in tandem with the T7 promoter for transcription. In this work, we describe the selection of T7 promoter variants of varying strength by emulsifying in vitro transcription with subsequent fluorescence activated cell sorting (FACS) to enrich for active promoters. Such variant promoters should be of use to synthetic biologists for both in vivo and in vitro applications.

Original languageEnglish (US)
Title of host publicationPacific Symposium on Biocomputing 2010, PSB 2010
Pages433-443
Number of pages11
StatePublished - 2010
Event15th Pacific Symposium on Biocomputing, PSB 2010 - Kamuela, HI, United States
Duration: Jan 4 2010Jan 8 2010

Other

Other15th Pacific Symposium on Biocomputing, PSB 2010
CountryUnited States
CityKamuela, HI
Period1/4/101/8/10

Fingerprint

Transcription
Emulsions
Biological systems
RNA
Sorting
Genes
Gene Regulatory Networks
Fluorescence
Cells
Networks (circuits)
Flow Cytometry
In Vitro Techniques

ASJC Scopus subject areas

  • Computational Theory and Mathematics
  • Biomedical Engineering
  • Medicine(all)

Cite this

Davidson, E. A., Van Blarcom, T., Levy, M., & Ellington, A. D. (2010). Emulsion based selection of T7 promoters of varying activity. In Pacific Symposium on Biocomputing 2010, PSB 2010 (pp. 433-443)

Emulsion based selection of T7 promoters of varying activity. / Davidson, Eric A.; Van Blarcom, Thomas; Levy, Matthew; Ellington, Andrew D.

Pacific Symposium on Biocomputing 2010, PSB 2010. 2010. p. 433-443.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Davidson, EA, Van Blarcom, T, Levy, M & Ellington, AD 2010, Emulsion based selection of T7 promoters of varying activity. in Pacific Symposium on Biocomputing 2010, PSB 2010. pp. 433-443, 15th Pacific Symposium on Biocomputing, PSB 2010, Kamuela, HI, United States, 1/4/10.
Davidson EA, Van Blarcom T, Levy M, Ellington AD. Emulsion based selection of T7 promoters of varying activity. In Pacific Symposium on Biocomputing 2010, PSB 2010. 2010. p. 433-443
Davidson, Eric A. ; Van Blarcom, Thomas ; Levy, Matthew ; Ellington, Andrew D. / Emulsion based selection of T7 promoters of varying activity. Pacific Symposium on Biocomputing 2010, PSB 2010. 2010. pp. 433-443
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