Embryonic stem cells release potentially novel hematopoietic factors

Helena Croizat, Eric E. Bouhassira

Research output: Contribution to journalArticle

Abstract

We have observed that a severe decrease in the number of erythroid progenitor cells follows the long-term culture (LTC) of human primitive stem cells on an established mouse fibroblast feeder layer. This suggests that one, or several, factors necessary to support erythroid differentiation might be missing in these culture conditions. To improve the erythroid differentiation and stem cell output of LTCs, we have examined the hypothesis that the factors that regulate pluripotent stem cell proliferation and erythroid commitment can be found in media conditioned by embryonic stem (ES) cells. We have found that media conditioned by undifferentiated and differentiating ES cells can affect differentiation patterns in both short-term culture and LTC assays. Medium conditioned by undifferentiated ES cells increases the numbers of estimated LTC-initiating cells (est.LTC-IC) and potentiates granulocytic differentiation. In contrast, medium conditioned by ES cells undergoing differentiation increases the number of est.LTC-IC and is a powerful promoter of erythroid differentiation. In the presence of this medium, the number of erythroid progenitors generated after 5 weeks of LTC was increased up to 100-fold as compared with controls, and the number of est.LTC-IC was amplified up to 140-fold. These results offer a new approach for the identification of factors implicated in stem cell proliferation, self-renewal and commitment. In addition, the improved LTC conditions for the expansion of stem cells without reduction of their in vitro differentiation potential should be useful for a wide range of appIications. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalActa Haematologica
Volume102
Issue number4
StatePublished - 1999

Fingerprint

Embryonic Stem Cells
Conditioned Culture Medium
Erythroid Precursor Cells
Stem Cells
Cell Proliferation
Feeder Cells
Pluripotent Stem Cells
Cell Differentiation
Cell Culture Techniques
Fibroblasts
Cell Count

Keywords

  • ES cells
  • Growth factors
  • Hematopoiesis
  • Long-term culture
  • Stem cells

ASJC Scopus subject areas

  • Hematology

Cite this

Embryonic stem cells release potentially novel hematopoietic factors. / Croizat, Helena; Bouhassira, Eric E.

In: Acta Haematologica, Vol. 102, No. 4, 1999, p. 172-179.

Research output: Contribution to journalArticle

@article{c0d9190148eb435e8c003abe9317a9fa,
title = "Embryonic stem cells release potentially novel hematopoietic factors",
abstract = "We have observed that a severe decrease in the number of erythroid progenitor cells follows the long-term culture (LTC) of human primitive stem cells on an established mouse fibroblast feeder layer. This suggests that one, or several, factors necessary to support erythroid differentiation might be missing in these culture conditions. To improve the erythroid differentiation and stem cell output of LTCs, we have examined the hypothesis that the factors that regulate pluripotent stem cell proliferation and erythroid commitment can be found in media conditioned by embryonic stem (ES) cells. We have found that media conditioned by undifferentiated and differentiating ES cells can affect differentiation patterns in both short-term culture and LTC assays. Medium conditioned by undifferentiated ES cells increases the numbers of estimated LTC-initiating cells (est.LTC-IC) and potentiates granulocytic differentiation. In contrast, medium conditioned by ES cells undergoing differentiation increases the number of est.LTC-IC and is a powerful promoter of erythroid differentiation. In the presence of this medium, the number of erythroid progenitors generated after 5 weeks of LTC was increased up to 100-fold as compared with controls, and the number of est.LTC-IC was amplified up to 140-fold. These results offer a new approach for the identification of factors implicated in stem cell proliferation, self-renewal and commitment. In addition, the improved LTC conditions for the expansion of stem cells without reduction of their in vitro differentiation potential should be useful for a wide range of appIications. Copyright (C) 2000 S. Karger AG, Basel.",
keywords = "ES cells, Growth factors, Hematopoiesis, Long-term culture, Stem cells",
author = "Helena Croizat and Bouhassira, {Eric E.}",
year = "1999",
language = "English (US)",
volume = "102",
pages = "172--179",
journal = "Acta Haematologica",
issn = "0001-5792",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Embryonic stem cells release potentially novel hematopoietic factors

AU - Croizat, Helena

AU - Bouhassira, Eric E.

PY - 1999

Y1 - 1999

N2 - We have observed that a severe decrease in the number of erythroid progenitor cells follows the long-term culture (LTC) of human primitive stem cells on an established mouse fibroblast feeder layer. This suggests that one, or several, factors necessary to support erythroid differentiation might be missing in these culture conditions. To improve the erythroid differentiation and stem cell output of LTCs, we have examined the hypothesis that the factors that regulate pluripotent stem cell proliferation and erythroid commitment can be found in media conditioned by embryonic stem (ES) cells. We have found that media conditioned by undifferentiated and differentiating ES cells can affect differentiation patterns in both short-term culture and LTC assays. Medium conditioned by undifferentiated ES cells increases the numbers of estimated LTC-initiating cells (est.LTC-IC) and potentiates granulocytic differentiation. In contrast, medium conditioned by ES cells undergoing differentiation increases the number of est.LTC-IC and is a powerful promoter of erythroid differentiation. In the presence of this medium, the number of erythroid progenitors generated after 5 weeks of LTC was increased up to 100-fold as compared with controls, and the number of est.LTC-IC was amplified up to 140-fold. These results offer a new approach for the identification of factors implicated in stem cell proliferation, self-renewal and commitment. In addition, the improved LTC conditions for the expansion of stem cells without reduction of their in vitro differentiation potential should be useful for a wide range of appIications. Copyright (C) 2000 S. Karger AG, Basel.

AB - We have observed that a severe decrease in the number of erythroid progenitor cells follows the long-term culture (LTC) of human primitive stem cells on an established mouse fibroblast feeder layer. This suggests that one, or several, factors necessary to support erythroid differentiation might be missing in these culture conditions. To improve the erythroid differentiation and stem cell output of LTCs, we have examined the hypothesis that the factors that regulate pluripotent stem cell proliferation and erythroid commitment can be found in media conditioned by embryonic stem (ES) cells. We have found that media conditioned by undifferentiated and differentiating ES cells can affect differentiation patterns in both short-term culture and LTC assays. Medium conditioned by undifferentiated ES cells increases the numbers of estimated LTC-initiating cells (est.LTC-IC) and potentiates granulocytic differentiation. In contrast, medium conditioned by ES cells undergoing differentiation increases the number of est.LTC-IC and is a powerful promoter of erythroid differentiation. In the presence of this medium, the number of erythroid progenitors generated after 5 weeks of LTC was increased up to 100-fold as compared with controls, and the number of est.LTC-IC was amplified up to 140-fold. These results offer a new approach for the identification of factors implicated in stem cell proliferation, self-renewal and commitment. In addition, the improved LTC conditions for the expansion of stem cells without reduction of their in vitro differentiation potential should be useful for a wide range of appIications. Copyright (C) 2000 S. Karger AG, Basel.

KW - ES cells

KW - Growth factors

KW - Hematopoiesis

KW - Long-term culture

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=0033507270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033507270&partnerID=8YFLogxK

M3 - Article

C2 - 10725758

AN - SCOPUS:0033507270

VL - 102

SP - 172

EP - 179

JO - Acta Haematologica

JF - Acta Haematologica

SN - 0001-5792

IS - 4

ER -