Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system

Mukesh K. Jain, Saori Kashiki, Chung Ming Hsieh, Matthew D. Layne, Shaw Fang Yet, Nicholas E.S. Sibinga, Michael T. Chin, Mark W. Feinberg, Ian Woo, Richard L. Maas, Edgar Haber, Mu En Lee

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Proteins of the LIM family are critical regulators of development and differentiation in various cell types. We have described the cloning of cysteine-rich protein 2/smooth muscle LIM protein (CRP2/SmLIM), a LIM-only protein expressed in differentiated vascular smooth muscle cells. As a first step toward understanding the potential functions of CRP2/SmLIM, we analyzed its expression after gastrulation in developing mice and compared the expression of CRP2/SmLIM with that of the other 2 members of the CRP subclass, CRP1 and CRP3/MLP. In situ hybridization in whole-mount and sectioned embryos showed that CRP2/SmLIM was expressed in the sinus venosus and the 2 cardiac Chambers at embryonic day 9. Vascular expression of CRP2/SmLIM was first seen at embryonic day 10. At subsequent time points, CRP2/SmLIM expression decreased in the heart but remained high in the vasculature. CRP1 was expressed both in vascular and nonvascular tissues containing smooth muscle cells, whereas CRP3/MLP was expressed only in tissues containing striated muscle. These patterns of expression were maintained in the adult animal and suggest an important role for this gene family in the development of smooth and striated muscle.

Original languageEnglish (US)
Pages (from-to)980-985
Number of pages6
JournalCirculation Research
Volume83
Issue number10
StatePublished - Nov 16 1998
Externally publishedYes

Fingerprint

Cardiovascular System
Cysteine
Smooth Muscle
Proteins
Striated Muscle
Smooth Muscle Myocytes
Blood Vessels
Gastrulation
cysteine and glycine-rich protein 3
Vascular Smooth Muscle
In Situ Hybridization
Organism Cloning
Embryonic Structures
Genes

Keywords

  • Development, mouse hybridization, in situ
  • Muscle, cardiac
  • Muscle, smooth
  • Protein, zinc-finger

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Jain, M. K., Kashiki, S., Hsieh, C. M., Layne, M. D., Yet, S. F., Sibinga, N. E. S., ... Lee, M. E. (1998). Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system. Circulation Research, 83(10), 980-985.

Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system. / Jain, Mukesh K.; Kashiki, Saori; Hsieh, Chung Ming; Layne, Matthew D.; Yet, Shaw Fang; Sibinga, Nicholas E.S.; Chin, Michael T.; Feinberg, Mark W.; Woo, Ian; Maas, Richard L.; Haber, Edgar; Lee, Mu En.

In: Circulation Research, Vol. 83, No. 10, 16.11.1998, p. 980-985.

Research output: Contribution to journalArticle

Jain, MK, Kashiki, S, Hsieh, CM, Layne, MD, Yet, SF, Sibinga, NES, Chin, MT, Feinberg, MW, Woo, I, Maas, RL, Haber, E & Lee, ME 1998, 'Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system', Circulation Research, vol. 83, no. 10, pp. 980-985.
Jain, Mukesh K. ; Kashiki, Saori ; Hsieh, Chung Ming ; Layne, Matthew D. ; Yet, Shaw Fang ; Sibinga, Nicholas E.S. ; Chin, Michael T. ; Feinberg, Mark W. ; Woo, Ian ; Maas, Richard L. ; Haber, Edgar ; Lee, Mu En. / Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system. In: Circulation Research. 1998 ; Vol. 83, No. 10. pp. 980-985.
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