Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy

Fran Adar, Linda A. Jelicks, Coralie Naudin, Denis Rousseau, Syun Ru Yeh

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

Raman and FTIR microprobe spectroscopy have been used to characterize the atherosclerotic process in Apo E and wild type mice. The Apo E null mouse is being studied in parallel with a healthy strain as a model of the human atherosclerotic disease. Preliminary Raman microprobe spectra have been recorded from the lumen of the aorta vessels from a normal black mouse (C57BL/6J) and the apo E null mouse fed on a normal chow diet. Spectra were also recorded from another normal mouse fed breeder chow containing a much higher content of fats. In the Raman spectra the fat cells exhibited spectra typical of esterified triglycerides while the wall tissue had spectra dominated by Amide I and III modes and the phenylalanine stretch at 1003 cm -1 of protein. The FTIR spectra showed the typical Amide I and II bands of protein and the strong >C=O stretch of the triglycerides. In addition, there were morphologically distinct regions of the specimens indicating a surprising form of calcification in one very old mouse (wild type), and free fatty acid inclusions in the knock out mouse. The observation of these chemistries provide new information for elucidation of the molecular mechanisms of the development of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)102-110
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5321
DOIs
StatePublished - Oct 27 2004
EventProgress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies - San Jose, CA, United States
Duration: Jan 25 2004Jan 27 2004

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy'. Together they form a unique fingerprint.

Cite this