Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy

Fran Adar, Linda Jelicks, Coralie Naudin, Denis L. Rousseau, Syun-Ru Yeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Raman and FTIR microprobe spectroscopy have been used to characterize the atherosclerotic process in Apo E and wild type mice. The Apo E null mouse is being studied in parallel with a healthy strain as a model of the human atherosclerotic disease. Preliminary Raman microprobe spectra have been recorded from the lumen of the aorta vessels from a normal black mouse (C57BL/6J) and the apo E null mouse fed on a normal chow diet. Spectra were also recorded from another normal mouse fed breeder chow containing a much higher content of fats. In the Raman spectra the fat cells exhibited spectra typical of esterified triglycerides while the wall tissue had spectra dominated by Amide I and III modes and the phenylalanine stretch at 1003 cm -1 of protein. The FTIR spectra showed the typical Amide I and II bands of protein and the strong >C=O stretch of the triglycerides. In addition, there were morphologically distinct regions of the specimens indicating a surprising form of calcification in one very old mouse (wild type), and free fatty acid inclusions in the knock out mouse. The observation of these chemistries provide new information for elucidation of the molecular mechanisms of the development of atherosclerosis.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsA. Mahadevan-Jansen, M.G. Sowa, G.J. Puppels, Z. Gryczynski, T. Vo-Dinh, J.R. Lakowicz
Pages102-110
Number of pages9
Volume5321
DOIs
StatePublished - 2004
EventProgress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies - San Jose, CA, United States
Duration: Jan 25 2004Jan 27 2004

Other

OtherProgress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies
CountryUnited States
CitySan Jose, CA
Period1/25/041/27/04

Fingerprint

Vibrational spectroscopy
Oils and fats
Amides
mice
Proteins
Nutrition
Fatty acids
spectroscopy
Raman scattering
fats
Spectroscopy
Tissue
amides
knockout mice
Raman spectra
proteins
arteriosclerosis
calcification
aorta
diets

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Adar, F., Jelicks, L., Naudin, C., Rousseau, D. L., & Yeh, S-R. (2004). Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy. In A. Mahadevan-Jansen, M. G. Sowa, G. J. Puppels, Z. Gryczynski, T. Vo-Dinh, & J. R. Lakowicz (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5321, pp. 102-110) https://doi.org/10.1117/12.529308

Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy. / Adar, Fran; Jelicks, Linda; Naudin, Coralie; Rousseau, Denis L.; Yeh, Syun-Ru.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / A. Mahadevan-Jansen; M.G. Sowa; G.J. Puppels; Z. Gryczynski; T. Vo-Dinh; J.R. Lakowicz. Vol. 5321 2004. p. 102-110.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Adar, F, Jelicks, L, Naudin, C, Rousseau, DL & Yeh, S-R 2004, Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy. in A Mahadevan-Jansen, MG Sowa, GJ Puppels, Z Gryczynski, T Vo-Dinh & JR Lakowicz (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5321, pp. 102-110, Progress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, San Jose, CA, United States, 1/25/04. https://doi.org/10.1117/12.529308
Adar F, Jelicks L, Naudin C, Rousseau DL, Yeh S-R. Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy. In Mahadevan-Jansen A, Sowa MG, Puppels GJ, Gryczynski Z, Vo-Dinh T, Lakowicz JR, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5321. 2004. p. 102-110 https://doi.org/10.1117/12.529308
Adar, Fran ; Jelicks, Linda ; Naudin, Coralie ; Rousseau, Denis L. ; Yeh, Syun-Ru. / Elucidation of the atherosclerotic disease process in apo E and wild type mice by vibrational spectroscopy. Proceedings of SPIE - The International Society for Optical Engineering. editor / A. Mahadevan-Jansen ; M.G. Sowa ; G.J. Puppels ; Z. Gryczynski ; T. Vo-Dinh ; J.R. Lakowicz. Vol. 5321 2004. pp. 102-110
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