Elongation factor-1α is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma

Brian T. Edmonds, Jeffrey Wyckoff, Yee Guide Yeung, Yun Wang, E. Richard Stanley, Joan Jones, Jeffrey E. Segall, John S. Condeelis

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Overexpression of elongation factor-1α (EF1α) mRNA has been correlated with increased metastatic potential in mammary adenocarcinoma; however, this relationship was not explored at the level of protein expression. As EF1α has been shown in other cell types to be a component of the actin cytoskeleton, a likely effector in metastasis, the actin binding activity of EF1α from metastatic and nonmetastatic rat breast tumors and cell lines was investigated. We have shown that EF1α protein is overexpressed in metastatic compared to nonmetastatic cells and whole tumors. Similarly to other EF1αs, both types of tumor EF1α bind to F-actin, but EF1α from metastatic cells has a reduced affinity for actin. In addition, there is a high correlation between the intracellular distribution of filamentous actin and EF1α in those cytoskeletal structures thought to be important for supporting the cellular motility required for metastasis. Following stimulation with EGF, there is a parallel increase in the amount of F-actin and EF1α associated with the cytoskeleton. The response to EGF can be blocked with cytochalasin D indicating that the binding of EF1α to the cytoskeleton is mediated by F-actin. We propose that a weakened association of EF1α with actin may be related to the metastatic process via an altered organization of the actin cytoskeleton and the differential translation of mRNAs associated with the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)2705-2714
Number of pages10
JournalJournal of Cell Science
Volume109
Issue number11
StatePublished - Nov 1996

Fingerprint

Peptide Elongation Factor 1
Microfilament Proteins
Adenocarcinoma
Breast
Actins
Cytoskeleton
Actin Cytoskeleton
Epidermal Growth Factor
Neoplasm Metastasis
Cytochalasin D
Protein Biosynthesis
Tumor Cell Line
Neoplasms
Proteins
Breast Neoplasms

Keywords

  • Cell motility
  • Chemotaxis
  • Cytoskeleton
  • EGF
  • Protein synthesis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Edmonds, B. T., Wyckoff, J., Yeung, Y. G., Wang, Y., Stanley, E. R., Jones, J., ... Condeelis, J. S. (1996). Elongation factor-1α is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma. Journal of Cell Science, 109(11), 2705-2714.

Elongation factor-1α is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma. / Edmonds, Brian T.; Wyckoff, Jeffrey; Yeung, Yee Guide; Wang, Yun; Stanley, E. Richard; Jones, Joan; Segall, Jeffrey E.; Condeelis, John S.

In: Journal of Cell Science, Vol. 109, No. 11, 11.1996, p. 2705-2714.

Research output: Contribution to journalArticle

Edmonds, BT, Wyckoff, J, Yeung, YG, Wang, Y, Stanley, ER, Jones, J, Segall, JE & Condeelis, JS 1996, 'Elongation factor-1α is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma', Journal of Cell Science, vol. 109, no. 11, pp. 2705-2714.
Edmonds BT, Wyckoff J, Yeung YG, Wang Y, Stanley ER, Jones J et al. Elongation factor-1α is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma. Journal of Cell Science. 1996 Nov;109(11):2705-2714.
Edmonds, Brian T. ; Wyckoff, Jeffrey ; Yeung, Yee Guide ; Wang, Yun ; Stanley, E. Richard ; Jones, Joan ; Segall, Jeffrey E. ; Condeelis, John S. / Elongation factor-1α is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma. In: Journal of Cell Science. 1996 ; Vol. 109, No. 11. pp. 2705-2714.
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