Elemental bioimaging of manganese uptake in C. elegans

Sigrid Große Brinkhaus, Julia Bornhorst, Sudipta Chakraborty, Christoph A. Wehe, Rebecca Niehaus, Olga Reifschneider, Michael Aschner, Uwe Karst

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A new method for elemental bioimaging with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed and applied to study the uptake of manganese (Mn) in Caenorhabditis elegans (C. elegans). C. elegans is a well-established model organism in neuroscience, genetics and genomics, which has been extensively studied to decipher mechanisms of heavy metal induced toxicity. Knowledge about the distribution of manganese (Mn) and other metals in this organism will be helpful in elucidating pathways and mechanisms of transport, distribution and excretion. The LA-ICP-MS method requires limited sample preparation and can be used rapidly and easily to visualize the Mn distribution in C. elegans. Due to thorough optimization of the analytical parameters, intense Mn signals in C. elegans wild-type (WT) and mutants were obtained at a spatial resolution as small as 4 μm, thus proving the suitability of LA-ICP-MS to study the uptake of metals in C. elegans.

Original languageEnglish (US)
Pages (from-to)617-621
Number of pages5
JournalMetallomics
Volume6
Issue number3
DOIs
StatePublished - Mar 2014
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Manganese
Inductively coupled plasma mass spectrometry
Laser ablation
Laser Therapy
Mass Spectrometry
Metals
Heavy Metals
Heavy metals
Toxicity
Neurosciences
Genomics

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics

Cite this

Große Brinkhaus, S., Bornhorst, J., Chakraborty, S., Wehe, C. A., Niehaus, R., Reifschneider, O., ... Karst, U. (2014). Elemental bioimaging of manganese uptake in C. elegans. Metallomics, 6(3), 617-621. https://doi.org/10.1039/c3mt00334e

Elemental bioimaging of manganese uptake in C. elegans. / Große Brinkhaus, Sigrid; Bornhorst, Julia; Chakraborty, Sudipta; Wehe, Christoph A.; Niehaus, Rebecca; Reifschneider, Olga; Aschner, Michael; Karst, Uwe.

In: Metallomics, Vol. 6, No. 3, 03.2014, p. 617-621.

Research output: Contribution to journalArticle

Große Brinkhaus, S, Bornhorst, J, Chakraborty, S, Wehe, CA, Niehaus, R, Reifschneider, O, Aschner, M & Karst, U 2014, 'Elemental bioimaging of manganese uptake in C. elegans', Metallomics, vol. 6, no. 3, pp. 617-621. https://doi.org/10.1039/c3mt00334e
Große Brinkhaus S, Bornhorst J, Chakraborty S, Wehe CA, Niehaus R, Reifschneider O et al. Elemental bioimaging of manganese uptake in C. elegans. Metallomics. 2014 Mar;6(3):617-621. https://doi.org/10.1039/c3mt00334e
Große Brinkhaus, Sigrid ; Bornhorst, Julia ; Chakraborty, Sudipta ; Wehe, Christoph A. ; Niehaus, Rebecca ; Reifschneider, Olga ; Aschner, Michael ; Karst, Uwe. / Elemental bioimaging of manganese uptake in C. elegans. In: Metallomics. 2014 ; Vol. 6, No. 3. pp. 617-621.
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