Electron tomography methods for c. Elegans

David H. Hall, William J. Rice

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fi ducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages141-158
Number of pages18
Volume1327
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1327
ISSN (Print)10643745

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Keywords

  • Annotation
  • Back- projection
  • Denoising
  • Electron tomogram
  • Fiducial marker
  • High pressure freezing
  • Image acquisition
  • Image alignment
  • Markerless alignment

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hall, D. H., & Rice, W. J. (2015). Electron tomography methods for c. Elegans. In Methods in Molecular Biology (Vol. 1327, pp. 141-158). (Methods in Molecular Biology; Vol. 1327). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2842-2_11