TY - JOUR
T1 - Electron Microscopy and Three-Dimensional Image Reconstruction
AU - Hall, David H.
N1 - Funding Information:
through the National Institutes of Health (NIH). The director of the facility is Dr. Conly Rieder. The STERECON computer program operates there on a Sun workstation. The system can recognize structures semiautomatically in 2D, before stacking these images into 3D image files, with stereoscopic viewing capabilities.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - This chapter focuses on the electron microscopy studies of Caenorhabditis elegans. The chapter discusses the methods for three-dimensional (3D) image reconstruction, based largely on film techniques introduced by John White and Randle Ware. Digital imaging techniques seem poised to make 3D reconstruction more accessible and may simplify the exchange of morphological data between laboratories. Several specialized specimen preparation techniques for electron microscopy, including freeze fracture and electron microscopic immunocytochemistry are described. The chapter outlines the methods making it feasible to collect hundreds of micrographs of cells or cell processes from a serially sectioned nematode. After assembling an ordered collection of negatives, all produced at one magnification, it is desired to view them sequentially in a well-aligned “movie” through the specimen. It can be quickly comprehended and can trace the continuity of individual processes as they course through a nerve bundle from a movie, even when the registration of profiles from print to print is difficult to discern.
AB - This chapter focuses on the electron microscopy studies of Caenorhabditis elegans. The chapter discusses the methods for three-dimensional (3D) image reconstruction, based largely on film techniques introduced by John White and Randle Ware. Digital imaging techniques seem poised to make 3D reconstruction more accessible and may simplify the exchange of morphological data between laboratories. Several specialized specimen preparation techniques for electron microscopy, including freeze fracture and electron microscopic immunocytochemistry are described. The chapter outlines the methods making it feasible to collect hundreds of micrographs of cells or cell processes from a serially sectioned nematode. After assembling an ordered collection of negatives, all produced at one magnification, it is desired to view them sequentially in a well-aligned “movie” through the specimen. It can be quickly comprehended and can trace the continuity of individual processes as they course through a nerve bundle from a movie, even when the registration of profiles from print to print is difficult to discern.
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U2 - 10.1016/S0091-679X(08)61397-7
DO - 10.1016/S0091-679X(08)61397-7
M3 - Article
C2 - 8531736
AN - SCOPUS:0029447441
SN - 0091-679X
VL - 48
SP - 395
EP - 436
JO - Methods in Cell Biology
JF - Methods in Cell Biology
IS - C
ER -