Electron-microscopic study of the apical region of the toad bladder epithelial cell.

J. Sasaki, S. Tilles, John S. Condeelis, J. Carboni, L. Meiteles, N. Franki, R. Bolon, C. Robertson, R. M. Hays

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Antidiuretic hormone (ADH) promotes fusion of cytoplasmic tubules with the luminal membrane and delivery of particles from the tubules to the membrane. The particles are believed to be the water-conducting elements in the membrane. We have employed several scanning (SEM) and transmission electron-microscopic (TEM) techniques to study the relationship of the cytoplasmic tubules to the luminal membrane and to the apical cytoskeleton of the toad bladder epithelial cell. This paper reports the results of freeze-crack SEM and tannic acid-fixed TEM studies, as well as studies with a resinless method of embedding. Freeze-cracked epithelial cells reveal that the tubules are anchored in a matrix of cytoskeleton and granules just below the luminal membrane, and many, if not all, retain their anchorage to the matrix after ADH-induced fusion. Tannic acid-fixed specimens show that the tubules in unstimulated cells lie horizontally. Fusion appears to involve an angulation of the tubules, and this may be the major mode of ADH-induced tubule movement. There are suggestions in the tannic acid sections of filamentous attachments of tubules to the surrounding cytoskeleton. In addition there are prominent microfilament bundles running down the microvilli and a dense concentration of filaments just below the luminal membrane. The presence of these filaments is confirmed in the resinless sections, and their possible role in ADH action is discussed.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume247
Issue number3 Pt 1
StatePublished - Sep 1984

Fingerprint

Anura
Urinary Bladder
Epithelial Cells
Electrons
Vasopressins
Membranes
Tannins
Cytoskeleton
Microvilli
Actin Cytoskeleton
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sasaki, J., Tilles, S., Condeelis, J. S., Carboni, J., Meiteles, L., Franki, N., ... Hays, R. M. (1984). Electron-microscopic study of the apical region of the toad bladder epithelial cell. The American journal of physiology, 247(3 Pt 1).

Electron-microscopic study of the apical region of the toad bladder epithelial cell. / Sasaki, J.; Tilles, S.; Condeelis, John S.; Carboni, J.; Meiteles, L.; Franki, N.; Bolon, R.; Robertson, C.; Hays, R. M.

In: The American journal of physiology, Vol. 247, No. 3 Pt 1, 09.1984.

Research output: Contribution to journalArticle

Sasaki, J, Tilles, S, Condeelis, JS, Carboni, J, Meiteles, L, Franki, N, Bolon, R, Robertson, C & Hays, RM 1984, 'Electron-microscopic study of the apical region of the toad bladder epithelial cell.', The American journal of physiology, vol. 247, no. 3 Pt 1.
Sasaki, J. ; Tilles, S. ; Condeelis, John S. ; Carboni, J. ; Meiteles, L. ; Franki, N. ; Bolon, R. ; Robertson, C. ; Hays, R. M. / Electron-microscopic study of the apical region of the toad bladder epithelial cell. In: The American journal of physiology. 1984 ; Vol. 247, No. 3 Pt 1.
@article{b2e2af6307cd4b96a4c9157ad01a9c41,
title = "Electron-microscopic study of the apical region of the toad bladder epithelial cell.",
abstract = "Antidiuretic hormone (ADH) promotes fusion of cytoplasmic tubules with the luminal membrane and delivery of particles from the tubules to the membrane. The particles are believed to be the water-conducting elements in the membrane. We have employed several scanning (SEM) and transmission electron-microscopic (TEM) techniques to study the relationship of the cytoplasmic tubules to the luminal membrane and to the apical cytoskeleton of the toad bladder epithelial cell. This paper reports the results of freeze-crack SEM and tannic acid-fixed TEM studies, as well as studies with a resinless method of embedding. Freeze-cracked epithelial cells reveal that the tubules are anchored in a matrix of cytoskeleton and granules just below the luminal membrane, and many, if not all, retain their anchorage to the matrix after ADH-induced fusion. Tannic acid-fixed specimens show that the tubules in unstimulated cells lie horizontally. Fusion appears to involve an angulation of the tubules, and this may be the major mode of ADH-induced tubule movement. There are suggestions in the tannic acid sections of filamentous attachments of tubules to the surrounding cytoskeleton. In addition there are prominent microfilament bundles running down the microvilli and a dense concentration of filaments just below the luminal membrane. The presence of these filaments is confirmed in the resinless sections, and their possible role in ADH action is discussed.",
author = "J. Sasaki and S. Tilles and Condeelis, {John S.} and J. Carboni and L. Meiteles and N. Franki and R. Bolon and C. Robertson and Hays, {R. M.}",
year = "1984",
month = "9",
language = "English (US)",
volume = "247",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "3 Pt 1",

}

TY - JOUR

T1 - Electron-microscopic study of the apical region of the toad bladder epithelial cell.

AU - Sasaki, J.

AU - Tilles, S.

AU - Condeelis, John S.

AU - Carboni, J.

AU - Meiteles, L.

AU - Franki, N.

AU - Bolon, R.

AU - Robertson, C.

AU - Hays, R. M.

PY - 1984/9

Y1 - 1984/9

N2 - Antidiuretic hormone (ADH) promotes fusion of cytoplasmic tubules with the luminal membrane and delivery of particles from the tubules to the membrane. The particles are believed to be the water-conducting elements in the membrane. We have employed several scanning (SEM) and transmission electron-microscopic (TEM) techniques to study the relationship of the cytoplasmic tubules to the luminal membrane and to the apical cytoskeleton of the toad bladder epithelial cell. This paper reports the results of freeze-crack SEM and tannic acid-fixed TEM studies, as well as studies with a resinless method of embedding. Freeze-cracked epithelial cells reveal that the tubules are anchored in a matrix of cytoskeleton and granules just below the luminal membrane, and many, if not all, retain their anchorage to the matrix after ADH-induced fusion. Tannic acid-fixed specimens show that the tubules in unstimulated cells lie horizontally. Fusion appears to involve an angulation of the tubules, and this may be the major mode of ADH-induced tubule movement. There are suggestions in the tannic acid sections of filamentous attachments of tubules to the surrounding cytoskeleton. In addition there are prominent microfilament bundles running down the microvilli and a dense concentration of filaments just below the luminal membrane. The presence of these filaments is confirmed in the resinless sections, and their possible role in ADH action is discussed.

AB - Antidiuretic hormone (ADH) promotes fusion of cytoplasmic tubules with the luminal membrane and delivery of particles from the tubules to the membrane. The particles are believed to be the water-conducting elements in the membrane. We have employed several scanning (SEM) and transmission electron-microscopic (TEM) techniques to study the relationship of the cytoplasmic tubules to the luminal membrane and to the apical cytoskeleton of the toad bladder epithelial cell. This paper reports the results of freeze-crack SEM and tannic acid-fixed TEM studies, as well as studies with a resinless method of embedding. Freeze-cracked epithelial cells reveal that the tubules are anchored in a matrix of cytoskeleton and granules just below the luminal membrane, and many, if not all, retain their anchorage to the matrix after ADH-induced fusion. Tannic acid-fixed specimens show that the tubules in unstimulated cells lie horizontally. Fusion appears to involve an angulation of the tubules, and this may be the major mode of ADH-induced tubule movement. There are suggestions in the tannic acid sections of filamentous attachments of tubules to the surrounding cytoskeleton. In addition there are prominent microfilament bundles running down the microvilli and a dense concentration of filaments just below the luminal membrane. The presence of these filaments is confirmed in the resinless sections, and their possible role in ADH action is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0021489488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021489488&partnerID=8YFLogxK

M3 - Article

VL - 247

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 3 Pt 1

ER -