Electrical synaptic transmission in developing zebrafish: Properties and molecular composition of gap junctions at a central auditory synapse

Cong Yao, Kimberly G. Vanderpool, Matthew Delfiner, Vanessa Eddy, Lexander G. Lucaci, Carolina Soto-Riveros, Thomas Yasumura, John E. Rash, Alberto E. Pereda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In contrast to the knowledge of chemical synapses, little is known regarding the properties of gap junctionmediated electrical synapses in developing zebrafish, which provide a valuable model to study neural function at the systems level. Identifiable “mixed” (electrical and chemical) auditory synaptic contacts known as “club endings” on Mauthner cells (2 large reticulospinal neurons involved in tail-flip escape responses) allow exploration of electrical transmission in fish. Here, we show that paralleling the development of auditory responses, electrical synapses at these contacts become anatomically identifiable at day 3 postfertilization, reaching a number of∼6 between days 4 and 9. Furthermore, each terminal contains ∼18 gap junctions, representing between 2,000 and 3,000 connexon channels formed by the teleost homologs of mammalian connexin 36. Electrophysiological recordings revealed that gap junctions at each of these contacts are functional and that synaptic transmission has properties that are comparable with those of adult fish. Thus a surprisingly small number of mixed synapses are responsible for the acquisition of auditory responses by the Mauthner cells, and these are likely sufficient to support escape behaviors at early developmental stages.

Original languageEnglish (US)
Pages (from-to)2102-2113
Number of pages12
JournalJournal of Neurophysiology
Volume112
Issue number9
DOIs
StatePublished - Nov 1 2014

Fingerprint

Electrical Synapses
Gap Junctions
Zebrafish
Synaptic Transmission
Synapses
Fishes
Tail
Neurons

Keywords

  • Auditory development
  • Connexin 35
  • Connexin 36
  • Escape response
  • Mauthner cell

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Electrical synaptic transmission in developing zebrafish : Properties and molecular composition of gap junctions at a central auditory synapse. / Yao, Cong; Vanderpool, Kimberly G.; Delfiner, Matthew; Eddy, Vanessa; Lucaci, Lexander G.; Soto-Riveros, Carolina; Yasumura, Thomas; Rash, John E.; Pereda, Alberto E.

In: Journal of Neurophysiology, Vol. 112, No. 9, 01.11.2014, p. 2102-2113.

Research output: Contribution to journalArticle

Yao, Cong ; Vanderpool, Kimberly G. ; Delfiner, Matthew ; Eddy, Vanessa ; Lucaci, Lexander G. ; Soto-Riveros, Carolina ; Yasumura, Thomas ; Rash, John E. ; Pereda, Alberto E. / Electrical synaptic transmission in developing zebrafish : Properties and molecular composition of gap junctions at a central auditory synapse. In: Journal of Neurophysiology. 2014 ; Vol. 112, No. 9. pp. 2102-2113.
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