Electric charge distribution in proteins and ionic strength dependence of reaction rate

Tsuyoshi Egawa, Takahiro Masuda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ionic strength dependence of the reaction rate between protein and dichloride anion radical has been investigated by flash photolysis of aqueous chloride-containing lysozyme, ribonuclease A, or insulin. The rate constant for the reaction of lysozyme or ribonuclease A with dichloride anion radicals decreases with increasing ionic strength, while it increases for insulin. The dependence was found to obey an equation derived from the theory of Debye and Hückel or the equation of Wherland and Gray for lysozyme within experimental errors. For ribonuclease A, however, it deviates largely from these equations. In the case of insulin a moderate deviation was observed. The different behavior in the ionic strength dependence is discussed in terms of the electric charge distribution in the protein molecules.

Original languageEnglish (US)
Pages (from-to)207-213
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume995
Issue number3
DOIs
StatePublished - May 1 1989
Externally publishedYes

Fingerprint

Pancreatic Ribonuclease
Electric charge
Charge distribution
Muramidase
Ionic strength
Osmolar Concentration
Reaction rates
Insulin
Anions
Proteins
Photolysis
Chlorides
Rate constants
Molecules

Keywords

  • Anion radical
  • Electric charge distribution
  • Flash photolysis
  • Ionic strength dependence
  • Reaction rate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

Cite this

Electric charge distribution in proteins and ionic strength dependence of reaction rate. / Egawa, Tsuyoshi; Masuda, Takahiro.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 995, No. 3, 01.05.1989, p. 207-213.

Research output: Contribution to journalArticle

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