Egg-sperm interactions at fertilization in mammals

Paul M. Wassarman, Luca Jovine, Eveline S. Litscher, Huayu Qi, Zev Williams

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Mammalian eggs are surrounded by a zona pellucida (ZP) that regulates egg-sperm teractions during fertilization. The ZP consists of long filaments composed of two glycoproteins, ZP2 and ZP3, that are crosslinked by a third glycoprotein, ZP1. The presence of both ZP2 and ZP3 is essential for assembling a ZP around growing oocytes, as well as for fertility of females. Acrosome-intact sperm recognize and bind to O-linked oligosaccharides linked to Ser residues at the sperm combining-site of ZP3. Structural differences in oligosaccharides on ZP3 from different species may account for whether or not sperm are able to bind to the ZP. Bound sperm undergo the acrosome reaction, penetrate the ZP, and can then fuse with egg plasma membrane. Following fertilization, sperm are unable to bind to either ZP3 or the ZP of one-cell embryos.

Original languageEnglish (US)
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume115
Issue numberSUPPL.
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

Fingerprint

Sperm-Ovum Interactions
Zona Pellucida
Fertilization
Spermatozoa
Mammals
Oligosaccharides
Ovum
Glycoproteins
Acrosome Reaction
Acrosome
Eggs
Oocytes
Fertility
Embryonic Structures
Binding Sites
Cell Membrane

Keywords

  • Acrosome reaction
  • Eggs
  • Fertilization
  • Sperm
  • Zona pellucida
  • ZP glycoproteins

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Egg-sperm interactions at fertilization in mammals. / Wassarman, Paul M.; Jovine, Luca; Litscher, Eveline S.; Qi, Huayu; Williams, Zev.

In: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 115, No. SUPPL., 01.07.2004.

Research output: Contribution to journalArticle

Wassarman, Paul M. ; Jovine, Luca ; Litscher, Eveline S. ; Qi, Huayu ; Williams, Zev. / Egg-sperm interactions at fertilization in mammals. In: European Journal of Obstetrics Gynecology and Reproductive Biology. 2004 ; Vol. 115, No. SUPPL.
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