EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells

Jin Pu, Colin D. McCaig, Lin Cao, Zhiqiang Zhao, Jeffrey E. Segall, Min Zhao

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The mechanisms by which cancer cells migrate to metastasise are not fully understood. Breast cancers are accompanied by electrical depolarisation of tumour epithelial cells. The electrical changes can be detected on the skin and are used to differentiate malignant from benign breast tumours. Could the electrical signals play a role in metastasis by promoting tumour cell migration? We report that electric fields stimulate and direct migration of human breast cancer cells. Importantly, these effects were more significant in highly metastatic tumour cells than in low metastatic tumour cells. Electric-field-enhanced directional migration correlates well with the expression level of EGF receptor (EGFR/ErbB1). To confirm this, we transfected low metastatic clone MTC cells with human ErbB1, which signiflcantly increased the electrotactic response. Inhibition of ErbB1 completely abolished the directional response of MTLn3 cells to an electric field. Transfection of MTLn3 cells and NWA-MB-435 cells with expression vectors for. ErbB family members ErbB1, ErbB2 and ErbB3 also significantly enhanced EF-induced migration. These results suggest that electric signals might play a role in metastasis of breast cancers by enhancing cell migration through the ErbB-signalling pathway.

Original languageEnglish (US)
Pages (from-to)3395-3403
Number of pages9
JournalJournal of Cell Science
Volume120
Issue number19
DOIs
StatePublished - Oct 1 2007

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Epidermal Growth Factor Receptor
Breast Neoplasms
Neoplasms
Cell Movement
Neoplasm Metastasis
Transfection
Clone Cells
Epithelial Cells
Skin

Keywords

  • Breast cancer cells
  • Electric field
  • ErbBs
  • Migration
  • Tumour metastasis

ASJC Scopus subject areas

  • Cell Biology

Cite this

EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells. / Pu, Jin; McCaig, Colin D.; Cao, Lin; Zhao, Zhiqiang; Segall, Jeffrey E.; Zhao, Min.

In: Journal of Cell Science, Vol. 120, No. 19, 01.10.2007, p. 3395-3403.

Research output: Contribution to journalArticle

Pu, Jin ; McCaig, Colin D. ; Cao, Lin ; Zhao, Zhiqiang ; Segall, Jeffrey E. ; Zhao, Min. / EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells. In: Journal of Cell Science. 2007 ; Vol. 120, No. 19. pp. 3395-3403.
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