Efficient CNS gene delivery by intravenous injection

Jean Pierre Louboutin, Alena A. Chekmasova, Elena Marusich, Jayanta Roy-Chowdhury, David S. Strayer

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.

Original languageEnglish (US)
Pages (from-to)905-907
Number of pages3
JournalNature Methods
Volume7
Issue number11
DOIs
StatePublished - Nov 2010

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Neurology
Mannitol
Transgenes
Intravenous Injections
Central Nervous System
Genes
Intraperitoneal Injections
Neurons
Spinal Cord

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Louboutin, J. P., Chekmasova, A. A., Marusich, E., Roy-Chowdhury, J., & Strayer, D. S. (2010). Efficient CNS gene delivery by intravenous injection. Nature Methods, 7(11), 905-907. https://doi.org/10.1038/nmeth.1518

Efficient CNS gene delivery by intravenous injection. / Louboutin, Jean Pierre; Chekmasova, Alena A.; Marusich, Elena; Roy-Chowdhury, Jayanta; Strayer, David S.

In: Nature Methods, Vol. 7, No. 11, 11.2010, p. 905-907.

Research output: Contribution to journalArticle

Louboutin, JP, Chekmasova, AA, Marusich, E, Roy-Chowdhury, J & Strayer, DS 2010, 'Efficient CNS gene delivery by intravenous injection', Nature Methods, vol. 7, no. 11, pp. 905-907. https://doi.org/10.1038/nmeth.1518
Louboutin JP, Chekmasova AA, Marusich E, Roy-Chowdhury J, Strayer DS. Efficient CNS gene delivery by intravenous injection. Nature Methods. 2010 Nov;7(11):905-907. https://doi.org/10.1038/nmeth.1518
Louboutin, Jean Pierre ; Chekmasova, Alena A. ; Marusich, Elena ; Roy-Chowdhury, Jayanta ; Strayer, David S. / Efficient CNS gene delivery by intravenous injection. In: Nature Methods. 2010 ; Vol. 7, No. 11. pp. 905-907.
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