Efficient CNS gene delivery by intravenous injection

Jean Pierre Louboutin, Alena A. Chekmasova, Elena Marusich, J. Roy Chowdhury, David S. Strayer

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.

Original languageEnglish (US)
Pages (from-to)905-907
Number of pages3
JournalNature Methods
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2010

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Louboutin, J. P., Chekmasova, A. A., Marusich, E., Chowdhury, J. R., & Strayer, D. S. (2010). Efficient CNS gene delivery by intravenous injection. Nature Methods, 7(11), 905-907. https://doi.org/10.1038/nmeth.1518