Efficiency of the Pioneer Round of Translation Affects the Cellular Site of Nonsense-Mediated mRNA Decay

Hanae Sato, Nao Hosoda, Lynne E. Maquat

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

In mammalian cells, nonsense-mediated mRNA decay (NMD) is a consequence of nonsense codon recognition during a pioneer round of translation. This round can occur largely before or largely after the release of newly synthesized mRNA from nuclei, depending on the mRNA, and likely utilizes cytoplasmic ribosomes. We show that increasing the cellular concentration of the splicing factor SF2/ASF augments the efficiency of NMD and ultimately shifts NMD that takes place after mRNA export to the cytoplasm to NMD that occurs before mRNA release from nuclei. These changes are accompanied by an increased association of pioneer translation initiation complexes with SF2/ASF, translationally active ribosomes, and the translational activator TAP. Increased TAP binding correlates with increased SF2/ASF binding, but not increased REF/Aly or Y14 binding. Our results uncover an additional role for SF2/ASF and indicate that the efficiency of the pioneer round of translation influences the efficiency of subsequent rounds of translation.

Original languageEnglish (US)
Pages (from-to)255-262
Number of pages8
JournalMolecular Cell
Volume29
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Keywords

  • RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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